Preparation of poplar antibacterial compounds and application thereof as bactericidal agent

A compound, poplar technology, applied in the field of pesticides, can solve the problems of tracking and separating antibacterial compounds, which have not yet been seen, etc.

Inactive Publication Date: 2009-05-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Preparation of poplar antibacterial compounds and application thereof as bactericidal agent
  • Preparation of poplar antibacterial compounds and application thereof as bactericidal agent
  • Preparation of poplar antibacterial compounds and application thereof as bactericidal agent

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: Small-scale preparation of different polarity extracts of poplar

[0020] Take European and American 107 poplar branches, dry them indoors, grind them, take 900g, and use 95% ethanol (sample: ethanol = 1:5, g / mL) to reflux extract 3 times at 80°C, each time for 1h, and combine 3 The sub-ethanol extract was concentrated under reduced pressure to obtain a crude ethanol extract (60.81 g, yield 6.76%). Take 52.93 g of the ethanol crude extract, heat and dissolve with distilled water to make a suspension, extract with ethyl acetate and n-butanol in sequence, concentrate the ethyl acetate layer, n-butanol layer, and the last remaining water layer under reduced pressure to obtain Ethyl acetate fraction (32.95g, 4.21% yield), n-butanol fraction (10.53g, 1.34% yield) and water fraction (8.21g, 1.05% yield).

Embodiment 2

[0021] Example 2: Tracking separation and preparation of poplar antibacterial compounds

[0022] Take European and American 107 poplar branches, dry them indoors, crush them, take 5.79kg, and use 95% ethanol (sample: ethanol = 1:5, g / mL) to reflux extract at 80°C for 3 times, each time for 1h, and combine 3 times The ethanol extract was concentrated under reduced pressure to obtain a crude ethanol extract (430.16 g). Take 420.00 g of the ethanol crude extract, heat and dissolve it with distilled water to make a suspension, extract it with ethyl acetate and n-butanol successively, concentrate the ethyl acetate layer, n-butanol layer, and the last remaining water layer under reduced pressure to obtain Ethyl acetate fraction (236.32g), n-butanol fraction (135.70g) and water fraction (46.23g). For the ethyl acetate extraction part with better antibacterial activity, the active components were further tracked and separated, and normal pressure normal phase or reverse phase silica ...

Embodiment 3

[0023] Embodiment 3: Structural identification of fatty alkanol p-hydroxycinnamate (1)

[0024] A white powder (chloroform) was prepared according to the method described in Example 2. After developing a normal-phase silica gel plate with petroleum ether: acetone (2:1, v / v) system, a single dark spot was visible under 254nm ultraviolet light. (Rf=0.70), the sulfuric acid chromogen showed dark yellow spots.

[0025] 1 H NMR (CDCl 3 , 300MHz), aromatic region δ: 7.63ppm (1H, d, J = 15.96Hz) and 6.30ppm (1H, d, J = 15.96Hz) signals indicate that there is a trans double bond in the molecule; δ: 7.43ppm ( 2H, m) and 6.83ppm (2H, m) indicate that there is a para-substituted benzene ring in the molecule; the broad single peak at δ: 5.22ppm (1H, brs) indicates that there is a hydroxyl substitution in the benzene ring, 13 C NMR (CDCl 3 , 75MHz), δ: The signal of 157.5ppm also verified that there is a hydroxyl substitution in the benzene ring; 1 The proton signal in the H NMR high-...

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Abstract

The invention relates to preparation of a poplar antimicrobial compound and application thereof as an antimicrobial. The antimicrobial compounds are traced and separated from 107 branches of poplar in Europe and America; and through structural analysis, the antimicrobial compounds are para-hydroxy fat alkanol ester cinnamate, 5-hydroxy-7-methoxyflavone, 5, 7-ferrichrysin and 5, 7-dihydroxyflavanol. The invention first defines that flavone compounds are major antimicrobial compositions in the poplar, and simultaneously defines that extracts of the 107 poplars in Europe and America and antimicrobial compositions thereof have obvious inhibition activity to various phytopathogens, and can be used for prevention and treatment of insect pests and plant disease.

Description

technical field [0001] The invention relates to the preparation of a poplar compound with antibacterial activity, the structural identification of the compound and its antibacterial activity, and belongs to the technical field of pesticides. Background technique [0002] Poplar (Populus spp.) is a general term for plants of the genus Populus in the family Salicaceae. There are more than 60 species of poplar in China. In addition, there are many variants and introduced species. They are mainly distributed in the north and southwest regions and spread all over the country. They are rich in resources and are the main tree species for afforestation and afforestation in China. Poplar has the characteristics of drought resistance, cold resistance, fast growth, short rotation period, and high fiber quality. It plays a very important role in wind and sand fixation, environmental protection, water and soil conservation, and farmland improvement. It has many uses in industry and life....

Claims

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Application Information

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IPC IPC(8): C07D311/30C07C69/732C07C67/48A01N65/08A01P3/00
Inventor 周立刚陈源泉隋鹏彭友良王明安钟灵允周亚明黄永富
Owner CHINA AGRI UNIV
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