Recombinant clostridium and construction method and use thereof

A technology of Clostridium acetobutylicum and SMB-1, which is applied in the fields of application, botany equipment and methods, and microbial-based methods, and can solve problems such as not having the ability to compete with chemical synthesis
CN101423813BInactive Publication Date: 2010-12-22INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2010-12-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a recombinant clostridium, a method for constructing the same and application thereof. The method for constructing the recombinant clostridium is to introduce a gene for encoding formic dehydrogenase into a clostridium to construct a recombinant bacterium. The clostridium is an acetone butanol clostridium or a clostridium beijerinckii. The invention also discloses a methodfor producing butanol, which is to ferment and culture the recombinant bacterium constructed by the method for constructing the recombinant clostridium to produce the butanol. The yield of butanone produced through fermenting the recombinant acetone butanol clostridium constructed by the invention can reach as high as 14.1 grams per liter.
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Description

technical field

[0001] The invention relates to a recombinant clostridium and its construction method and application. Background technique

[0002] The non-renewability and limited storage of fossil energy is a major problem facing modern society. This has led to an upward trend in crude oil prices, which has placed a heavy burden on the country's economic development. The extensive use of fossil fuels has also brought about serious environmental problems. The burning of fossil fuels dramatically increases the amount of carbon dioxide in the atmosphere, causing the greenhouse effect and warming the global climate. In addition, the sulfide and nitrogen compounds emitted during the combustion of fossil fuels lead to acid rain on a large scale and damage the environment. Therefore, there is an urgent need to seek alternative and environmentally friendly renewable fuels. Biomass is an inexhaustible resource in nature, which is renewable and will not emit more carbon dioxide...

Claims

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