Method for separating and screening flocculation and demulsification double-function bacterial strain and culture medium used thereby

A technology of flocculation and demulsification, separation and screening, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of efficient treatment of oily sewage or oily sludge, long process time, and many equipments. Low cost, short process time, energy saving effect

Inactive Publication Date: 2009-05-20
HARBIN INST OF TECH
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  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of long process time, high energy consumption, large cost, and many equipments in the existing method for treating oily sewage or oily sludge that require engineering strains to be treated in two stages of demulsification and flocculation, and the two types of engineering strains cannot To deal with the problem of oily sewage or oily sludge efficiently in the same environment at the same time, and provide a method for separating and screening flocculation and demulsification bifunctional strains and the culture medium used

Method used

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  • Method for separating and screening flocculation and demulsification double-function bacterial strain and culture medium used thereby
  • Method for separating and screening flocculation and demulsification double-function bacterial strain and culture medium used thereby
  • Method for separating and screening flocculation and demulsification double-function bacterial strain and culture medium used thereby

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specific Embodiment approach 1

[0009] Specific embodiment one: the method for separating and screening flocculation and demulsification bifunctional bacterial strains in this embodiment is realized according to the following steps: one, carry out doubling dilution of collected samples under aerobic conditions to obtain doubling dilutions of different concentrations; The solid medium is melted in a boiling water bath, then cooled to a temperature of 45-55°C, and then the multiple dilutions of different concentrations are coated on the surface of the solid medium, and then cultured at a constant temperature at a temperature of 30-32°C Until there are colonies in the tube; 3. Pick a single colony and put it on the liquid medium, and then culture it at a constant temperature of 30-32°C for 2-3 days; 4. Separation and purification until pure strains appear, then they can be isolated and screened Flocculation and demulsification bifunctional strains are produced; wherein the solid medium in step 2 consists of 3.0g...

specific Embodiment approach 2

[0012] Embodiment 2: This embodiment differs from Embodiment 1 in that the sample collected in step 1 is water containing oil or soil containing oil. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0013] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 2, the temperature is cooled to 48-52°C. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a method for separating and filtering flocculating-demulsificating dual-functional strains and a culture medium that is used, relates to a strain separating and filtering method and a culture medium that is used, and solves the problems that existing oily sewage or oily sludge processing methods need two-stage processing of industrial strains that is divided into flocculating and demulsificating, has long process time, high energy consumption, high cost and more equipments, and two industrial strains cannot process oily sewage or oily sludge in high efficiency simultaneously in the same environment. The separating method comprises the following steps: 1. diluents with a plurality of proportions and different concentrations are prepared; 2. bacteria colonies are cultivated in a test tube; 3. individual bacterial colonies are selected and cultivated at constant temperature; and 4. separation and purification are implemented until the appearance of pure strains, and then the separation can be implemented. The separated and filtered flocculating-demulsificating dual-functional strains can simultaneously generate demulsificating and flocculating active ingredients, and are characterized by high demulsificating and flocculating rate. The culture medium that is used for filtering has strong directivity. The method for separating and filtering flocculating-demulsificating dual-functional strains has the advantages of short process time, saved energy, less cost and simple equipments.

Description

technical field [0001] The invention relates to a method for isolating and screening bacterial strains and the culture medium used therein. Background technique [0002] When using existing engineering strains to treat oily sewage or oily sludge, engineering strains with demulsification function and engineering strains with flocculation function are usually required to complete the treatment in two stages, so there are long process time, high energy consumption, and high cost. There are many problems with equipment. If the two types of engineering strains are placed in the same environment to treat oily sewage or oily sludge, although the above problems can be solved; but because the living conditions of the two types of engineering strains are bound to be different, it is inevitable to put the two types of engineering strains in the same environment. It cannot meet the survival needs of the two types of engineering strains at the same time, resulting in the reduction of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 马放侯宁李大鹏徐旸李絮魏利
Owner HARBIN INST OF TECH
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