Hemolytic agent

A technology of hemolytic agent and buffering agent, applied in the field of hemolytic agent, can solve problems such as the inability to guarantee stable and accurate measurement results, and achieve the effect of broadening the applicable temperature range, ensuring accuracy and consistency, and reducing requirements

Active Publication Date: 2009-06-24
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Most of the above existing technologies focus on the accuracy of hemoglobin measurement under normal temperature c...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A hemolytic agent having the following composition:

[0039] Dodecyltrimethylammonium Chloride 40g

[0040] Ascorbic acid 10g

[0041] Phosphate buffered saline 0.05M

[0042] Replenish water to 1L

[0043] pH 5.6

[0044]Use the BC-3000Plus liquid cell analyzer produced by Mindray Company, use the matching diluent provided by the manufacturer, and use the hemolytic agent of the above formula for automatic analysis. The specific process is carried out according to the method provided by the manufacturer, which specifically includes: mixing the whole blood and the diluent to form a diluted sample of the first concentration. The diluted sample of the first concentration was divided into two parts. A portion of the diluted sample of the first concentration is mixed with the diluent to form a sample of the second concentration, which is used for counting and measuring red blood cells and platelets. The remaining diluted sample of the first concentration is mixed with ...

Embodiment 2

[0051] A hemolytic agent having the following composition:

[0052] Dodecyltrimethylammonium Bromide 40g

[0053] p-Sulphanilic acid 10g

[0054] Phosphate buffered saline 0.05M

[0055] Replenish water to 1L

[0056] pH 7.6

[0057] Another blood sample was taken, and the hemolyzing agent of this example was used, and the rest were determined according to the method described in Example 1, and the results are shown in Table 2.

[0058] Table 2

[0059]

[0060] Results: The HGB deviation at 10°C compared to 25°C is 0.36%;

[0061] The HGB deviation at 40°C compared to 25°C is 2.7%

Embodiment 3

[0063] A hemolytic agent having the following composition:

[0064] Cetyltrimethylammonium Chloride 20g

[0065] Ascorbic acid 1g

[0066] Phosphate buffered saline 0.05M

[0067] Replenish water to 1L

[0068] pH 5.6

[0069] Another blood sample was taken, and the hemolyzing agent of this example was used, and the rest were determined according to the method described in Example 1, and the results are shown in Table 3.

[0070] table 3

[0071]

[0072] Results: The HGB deviation at 10°C compared to 25°C was 1.9%;

[0073] HGB deviation at 40°C compared to 25°C is 2.6%

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PUM

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Abstract

The invention discloses a hemolytic agent which contains at least one organic acid whose content ranges from 1 to 20g/L, and relates to a multipurpose hemolytic agent, which can be used for automatic hemanalysis, and can accurately test hematoglobin and keep the normal classification of leucocyte at the same time. A stabilizing agent of hematoglobin derivants is added in the hemolytic agent, thereby preventing the derivants from changing along with the reaction temperature change, and ensuring that an accurate and stable test result can be obtained within the normal service temperature range of the instrument.

Description

technical field [0001] The invention relates to a reagent for analyzing blood cells, in particular to a hemolyzing agent which can be used for leukocyte classification detection and hemoglobin content determination. Background technique [0002] Hemolytic reagent is one of the most commonly used reagents for blood cell analyzers, and it is also a key reagent for counting white blood cells and various groups, and determining HGB content. After the diluted blood is added with a hemolytic agent, the red blood cells are dissolved and hemoglobin is released. The latter combines with relevant components in the hemolytic agent to form a hemoglobin derivative, which enters the hemoglobin detection system. The change is proportional to the content of hemoglobin in the liquid, and the instrument can display its concentration; at the same time, different types of white blood cells (lymphocytes, monocytes, neutrophils, etc.) form obvious differences after the action of hemolytic agents....

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 张利娜刘牧龙许文娟
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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