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Miniature biochemical detection instrument with internal calibartion function and its matched reagent

A biochemical detector and detector technology, applied in the field of biological detectors, can solve the problems of high consumption, affect laboratory work efficiency, increase laboratory work intensity of medical institutions, etc., and achieve the effects of convenient use and accurate results.

Inactive Publication Date: 2009-06-24
SHANGHAI FOSUN PHARMA (GROUP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, on the one hand, medical institutions need to consume a large amount of calibrators / standards, quality control materials, reagents, etc., on the other hand, it also increases the work intensity of laboratories in medical institutions and affects the work efficiency of laboratories.

Method used

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  • Miniature biochemical detection instrument with internal calibartion function and its matched reagent
  • Miniature biochemical detection instrument with internal calibartion function and its matched reagent

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] figure 1 Shown is a schematic diagram of the planar structure of the small biochemical detector with internal calibration of the present invention. It is composed of a power supply 1 , an LED luminous tube 2 , a CPU processor 3 , a data printer 4 , a colorimetric cell 5 and operation keys 6 . The power supply 1 is located at the left rear of the analyzer, and it is connected with the LED luminous tube 2 , the CPU processor 3 , the data printer 4 , the colorimetric cell 5 and the operation keys 6 . The LED luminous tube 2 is located at the left front of the analyzer, and it is connected with the CPU processor 3 , the colorimetric cell 5 and the power supply 1 . The data printer 4 is located at the right rear of the analyzer, and it is connected with the CPU processor 3 and the power supply 1 . The CPU processor 3 is located at the center of the analyzer, and is connected with the power supply 1, the LED light-emitting tube 2, the data printer 4, the colorimetric cell 5...

example 2

[0027] Example 2 Glucose Detection Reagent

[0028] Reagent 1:

[0029] 1 liter of reagent contains

[0030] Phosphate buffered saline 34g

[0031] Peroxidase 375U / L

[0032] 4-Hydroxybenzoic acid 0.6 g

[0033] 4-aminopyrine 0.4 g

[0034] Sodium azide 1 g

[0035] Reagent 2:

[0036] 1 liter of reagent contains

[0037] Phosphate buffered saline 13.5 g

[0038] Glucose oxidase 30KU / L

[0039] Open the kit, modify the reaction parameters according to the marked reaction rate factor K and the reagent blank, and then put the reagent into the reagent chamber for sample determination. The reaction wavelength is 505nm, the reaction temperature is 37 degrees, the ratio of sample to reagent 1 and reagent 2 is 3:200:100, and the reaction time is 10 minutes. Read the change in absorbance 10 minutes after the sample reacts with the reagent.

[0040] The reagent blank of the batch on the glucose detection reagent is 0.3257, and the reaction rate factor K is 1211. The reagent...

example 3

[0041] Example 3 Bile acid detection reagent

[0042] Reagent 1:

[0043] 1 liter of reagent contains

[0044] Glycine 2.5 g

[0045] Thiooxidized nicotineurine 1.0g

[0046] Bovine Albumin 0.1g

[0047] Sodium azide 1 g

[0048] pH 4.3

[0049] Reagent 2:

[0050] 1 liter of reagent contains

[0051] Phosphate buffered saline 13.5 g

[0052] Reduced coenzyme 6 grams

[0053] 3α-Hydroxyalcohol Dehydrogenase 12KU

[0054] Bovine Albumin 0.1g

[0055] Sodium azide 1 g

[0056] pH 9.3

[0057] The detection wavelength of the total bile acid reagent is 405nm, the reaction rate factor of the previous batch of total bile acid reagent is 6557, the reagent blank is 0.2987, the reaction rate factor of this batch is 6513, and the reagent blank is 0.2864.

[0058] The last batch of reagents tested a sample and the change in absorbance per minute was 0.0532A, so the measured value was 0.0532×6557, which is 34.9 micromole / liter

[0059] This batch of reagents measured the sam...

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Abstract

The invention provides a miniature biochemical detector with internal calibration, which comprises the following parts: a power supply (1), an LED (2), a CPU (3), a data printer (4), a spectrophotometer-cell (5) and an operational key (6). The detector carries out self-detection on a light source system after starting up each time, and regulates working current automatically according to a detected value. The invention further provides a matching reagent used on the miniature biochemical detector with internal calibration.

Description

Technical field: [0001] The invention relates to a biological detector, in particular to a small biochemical detector with internal calibration and its supporting reagents. Background technique: [0002] At present, biochemical analyzers at home and abroad are developing rapidly and becoming more mature, such as Hitachi 7600, BECKMANLX20, BAY1650, Olympus AU1000 and so on. Although these instruments all have self-detection functions, they cannot realize self-correction. At the same time, due to the influence of various factors in the production process of reagent manufacturers, batch-to-batch differences will inevitably occur. For example, changes in the production environment, changes in the activity of raw materials, differences in feeding by operators, and batch-to-batch differences in raw materials will all cause batch-to-batch differences in reagents. If these differences are not calibrated, the accuracy of the detection results will be seriously affected. Therefore,...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/25
Inventor 张跃建
Owner SHANGHAI FOSUN PHARMA (GROUP) CO LTD