High-artificial tissue engineering nerve repair material NGCS and preparation method thereof
A technology for simulating tissue and nerve repair. It is used in medical science, prosthesis, etc. It can solve the problems of unable to maintain physical structure, affecting the effect of treatment or experiment, and unfavorable directional growth of regenerative fibers. It is beneficial to regeneration and improves mechanical properties. , the effect of increasing tensile strength and stiffness
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Embodiment 1
[0046] Embodiment 1: (according to type I collagen: chitosan: the ratio of gelatin is 280: 70: 70):
[0047] According to technical scheme of the present invention, taking collagen protein, gelatin and chitosan as example, its preparation method is carried out as follows:
[0048] 1). Weigh the above-mentioned type I collagen in parts by weight, put it into 3 mg / ml acetic acid solution at a concentration of 28 mg / ml, and dissolve it for 24 hours;
[0049] 2). Stir at 15000r / min for 90min in a constant temperature environment at 4°C to make a suspension;
[0050] 3). In addition, weigh chitosan and gelatin in the above weight ratio according to the ratio and add them to 3 mg / ml acetic acid solution to dissolve for 24 hours;
[0051] 4).Stir at 4°C and stir at 15000r / min for 90min to make a suspension;
[0052] 5). Mix the two suspensions and keep the constant temperature at 4°C, stir at 15000r / min for 90 minutes to make a suspension of collagen and chitosan, and leave it for ...
Embodiment 2
[0060] Embodiment 2: adopt collagen (type I), chitosan and gelatin as raw material, its weight is respectively 280mg, 280mg, 280mg, implementation process is the same as example 1.
Embodiment 3
[0061] Embodiment 3: adopt collagen (type I), chitosan and gelatin as raw material, its weight is respectively 280mg, 28mg, 28mg, implementation process is the same as example 1.
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