Method for cultivating unicellular algae by inflation
A single-cell algae and culture bottle technology, applied in the direction of single-cell algae, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., can solve the shortage of bait for shellfish larvae, increase the difficulty of management, and cultivate Environmental pollution and other issues, to achieve the effect of long duration, space saving and growth rate improvement
Inactive Publication Date: 2009-07-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology
Traditional primary and secondary cultures are usually cultivated in foam film bags, 50L plastic buckets and small cement tanks, the cultivation speed is slow and it is easy to pollute
It is generally believed that aerated culture can fully contact algae cells with the culture environment, thereby increasing the growth rate of algae cells, but because aeration is easy to pollute the culture environment, it also increases the difficulty of management, so it also has certain risks. Some The production unit adopts the traditional aerated culture method. Due to poor management and irregular operation, all algae species are often polluted, resulting in a serious shortage of bait for shellfish larvae, and even the failure of seedling cultivation.
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[0029] The method of the present invention and the conventional method (control group) were used for culturing respectively.
[0030] (1) Experimental method
[0031] 1.1 Experimental materials
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Abstract
The invention relates to the culture of unicellular algae, in particular to a method for the aerated culture of the unicellular algae. Under the conditions of 18-35 DEG C and natural lighting, aerated culture of unicellular algae is conducted in a culture flask filled with sand-filtered natural sea water; aeration quantity is 4-8L / min; the culture flask is filled with air by an inflator pump or a blower; and the air is filtered through 2-3 air filters, then exhausted to be dispersed in the transparent plastic culture flask and used for the first or second grade large-scale production and culture of unicellular algae which are bait of shellfish breeding. The invention has the advantages of convenient operation, stable and quick growth of algae and no pollution.
Description
technical field [0001] The present invention relates to the cultivation of algae, in particular to a method for culturing unicellular algae by aeration. Background technique [0002] Single-celled algae are the main bait in the process of shellfish nursery larvae cultivation, and are one of the key factors in determining shellfish nursery. However, in shellfish breeding, there are often insufficient and untimely bait supply, serious bait pollution in production ponds, and inability to supply algae in time, resulting in uncoordinated demand for bait growth and larvae. The unicellular algae culture method used in production generally includes the following links: seed preservation, primary culture, secondary culture, and tertiary culture (large pond culture). Traditional primary and secondary cultures are usually cultured in foam film bags, 50L plastic buckets and small cement ponds, which are slow and easily contaminated. It is generally believed that aeration culture can m...
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IPC IPC(8): C12N1/12C12M1/24
Inventor 毛玉泽方建光李锋朱玲于守团叶乃好杜美荣邹键
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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