Method for detecting nano material cell toxicity

Abstract

The invention relates to a cytotoxicity detecting method of nanophase materials and comprises the following steps: cells to be detected are inoculated on a plurality of pore plates with bottom micro-electronic sensors, and the pore plates are positioned on a detecting device of a cellular analyzing system; a cellular growth curve is monitored so as to obtain the adherence to microelectrode surfaces of the pore plates or growth situations of the cells; and after cell adherence is implemented for 2 to 30 hours, a sterilized culture fluid or buffer fluid of the nanophase materials is added, and the cellular growth curve continues to be monitored. The cytotoxicity detecting method of nanophase materials has the advantages of total automatic process, real-time monitoring, non-marking and non-damage of cells, can be used for detecting a plurality of objects, has small volume, less sample usage, low cost as well as simple and convenient operation of the detecting device, can be widely used for the toxicity detection of carbonic nanophase materials with high flux and high sensitivity, and raises analysis speed and easily realizes online control and detecting automation.

Description

technical field [0001] The invention relates to a method for detecting toxicity, in particular to a method for detecting cytotoxicity of nanomaterials. Background technique [0002] In recent years, the rapid development of nanotechnology and nanomaterials has attracted more and more attention. Nanomaterials refer to materials whose geometric dimensions reach the nanoscale level, including nanopowders, nanowires, nanofilms, and materials with special properties. When the size of the particle enters the nanometer level, it will show a strong small size effect, surface effect, quantum size and other effects. Compared with traditional materials, the properties of nanomaterials have changed greatly, and their biological effects have also occurred. qualitative change. Nanomaterials have entered our lives, even into the human body. However, there is a serious lack of information on the evaluation of the risk of nanomaterials. In fact, the governments and scientists of many cou...

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Problems solved by technology

These methods usually have high requirements on the experimental operators, the operation is complicated, the cells may be damaged during the operation, the sensitivity is low, the repeatability is poor, the time is long, the amount is large, the required equipment is large, and the cost is high.

At the same time, a large number of studies have found that there is a strong van der Waals interaction between the blue tetrazolium and the reduced formazan (Formazan), neutral red, porphyrin and other dye molecules and the carbon nanotube wall, and the electrons The microscope can observe that the dye molecules are adsorbed on the carbon tube wall, so that the indicator molecules cannot be accurately detected, which leads to inaccurate test results, and it is possible to get completely opposite results

[0004] From the above analysis, it can be seen that there are still many problems in the current methods of detecting the cytotoxicity of nanomaterials, and there are no detection methods that are conducive to market promotion.

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