Coumarin ether compounds and new use of composition
A technology of coumarin and compounds, applied in the field of pharmacy, can solve the problems of mitochondrial toxicity, easy drug resistance, and too expensive interferon
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Embodiment 1
[0073] The compound wedelolide extracted from Eclipta alba
[0074] (1) soaking and filtering
[0075] Eclipta whole grass 300kg, fully immersed in 0.75 tons of ethanol (concentration 95%)→soak overnight (10 hours)→coarse filtration, thereby removing the whole grass residue (retention)→net filtration (suction filtration) or high-speed centrifugation (10000rpm, 10 minutes), thereby removing dust and fine residue → green clarified filtrate.
[0077] Recover ethanol by distillation, the temperature does not exceed 60°C → distill for 2 hours each time, remove the extract in the reaction kettle to the collection bucket (the extract is dark dark green, slightly viscous) → repeat the above steps until all ethanol is recovered .
[0078] (3) Second soaking and back distillation
[0079] Recover 0.75 tons of ethanol, re-soak the whole herb residue → soak overnight, the requirements for coarse filtration, net filtration and distillation recovery are the...
Embodiment 2
[0092] Inhibitory effect of wedelolide on virus in vitro
[0093] Cells and viruses: herpes simplex virus type 1 (HSV-I), respiratory syncytial virus (RSV), poliovirus type 1 (Poliovirus-1), coxsackievirus type A-7 (CVA-7), ECHO-11 and Vero cells were purchased from the National Institute for the Control of Pharmaceutical and Biological Products, Ministry of Health.
[0094] Experimental drug: APL-1 (prepared in Example 1).
[0095] Experimental method: Vero cells were inoculated in 96-well cell culture plates, the number of cells was 25,000 / well, conventionally cultured with PRMI1640 medium for 24 hours, and 100TCID were added 50 For HSV-I, RSV, Poliovirus-1, CVA-7, ECHO-11, set 6 replicate wells for each virus, discard the virus solution after 2 hours and wash with Hanks solution for 3 times, then add the concentration of 1 μg / ml, 5 μg / ml, 10 μg / ml of APL-1, incubated at 37°C for 96 hours, and observed the degree of cytopathic changes in the cells of each group.
[0096]...
Embodiment 3
[0107] Antiviral effect of wedelolide in mice
[0108] Experimental animals: 70 male SPF grade BALB / C mice, purchased from Shanghai Slack Experimental Animal Co., Ltd., weighing 18-20 g. Raised in SPF grade animal room, 12h light / 12h dark, free intake of feed and water.
[0109] Virus strain: herpes simplex virus type I (HSV-1) Sm44 strain, purchased from National Institute for the Control of Pharmaceutical and Biological Products, Ministry of Health.
[0110] Experimental drugs: APL-1 (prepared in Example 1), ribavirin (ribavirin, purchased from Jiaozuo Kangli Pharmaceutical Co., Ltd., 0.1 g / ml).
[0111] Experimental method: Using different dilutions of the virus liquid, the experimental mice were inoculated intracranially with HSV-1 (TCID 50 =10 -7 ·ml -1 ), observed for 14 days. According to the number of mouse deaths, calculate the LD of the virus 50 , to determine the amount of virus inoculum. According to the probability unit method of half number effect, measure...
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