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Vaccine for fish and application

A vaccine composition and application technology, applied in skin diseases, bacterial antigen components, antibacterial drugs, etc.

Active Publication Date: 2009-08-05
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of Aeromonas caviae to prepare a whole-bacteria vaccine to prevent and treat the "ulcer syndrome" of the southern largemouth catfish

Method used

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  • Vaccine for fish and application
  • Vaccine for fish and application
  • Vaccine for fish and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0145] Embodiment 1 Preparation of whole bacteria vaccine of the present invention

[0146] 1. Materials and methods

[0147] 1.1 Materials

[0148] Test bacteria: Aeromonas caviae (A.caviae) isolated and identified from diseased largemouth catfish

[0149] Experimental fish: purchased from the Ya'an market, healthy, disease-free and injury-free, weighing about 200g, and kept for 7 days before the experiment.

[0150] Main reagents: Azone (chemically pure, produced by Chengdu Kelong Chemical Reagent Factory); racemic anisodamine hydrochloride injection (1ml: 10mg, 05072531, produced by Tianjin Pharmaceutical Jiaozuo Co., Ltd.). Tryptone, soybean peptone, nutrient agar, Beijing Boaoxing Biotechnology Co., Ltd.

[0151] Medium: TSA and TSB

[0152] 1.2 Method

[0153] 1.2.1 Feeding management

[0154] It is raised in an aquarium and continuously increases oxygen for 24 hours; the water source is tap water after aeration and dechlorination treatment, its pH value is 6.8-7.2...

Embodiment 2

[0183] Embodiment 2 The preparation and application method of vaccine preparation (aqueous) of the present invention

[0184] [Preparation of the vaccine]: Aeromonas caviae (DKN-1) was resuscitated and cultured, then primary cultured in TSB broth, then secondary cultured in TSA and enlarged cultured in TSB broth. Marin 28°C, 110r / min, shaking inactivation for 36h, after the inactivation is complete after inspection, aseptically bottled and ready for use. When used in combination with 10mg / l azone penetration enhancer, they can be mixed together or administered separately at the same time.

[0185] [Composition and shape]: This strain is an inactivated whole bacterin vaccine for southern largemouth catfish ulcer syndrome. The vaccine should be brownish-yellow, with white precipitate on standing.

[0186] [Specification]: The specification of this product is 500mL per bottle, and under normal circumstances, the bacteria content per milliliter is 10 10 Above CFU, when it canno...

Embodiment 3

[0196] Embodiment 3 The preparation and application method of the vaccine preparation (powder) of the present invention

[0197] 1. Vaccine Preparation

[0198] (1) Bacterial recovery

[0199] Under sterile conditions, take the strains stored at 4°C and use an inoculation loop to pick a small amount of bacteria from the strain tube to inoculate into 10ml TSB broth, put the inoculated broth into a water bath shaker, set at 28°C, 110r / min, shaking culture for 24h.

[0200] (2) Proliferation and culture of bacteria liquid

[0201] ①Primary training

[0202] Inoculate 10ml of the revived cultured bacterial solution and inoculate 500ml TSB broth again, put the inoculated broth into a water bath shaker, 28°C, 110r / min, and shake for 24h.

[0203] ②Secondary culture of bacterial liquid

[0204] Spread the secondary cultured TSB broth evenly on the prepared ordinary nutrient agar plate, put the coated ordinary plate into a constant temperature incubator at 28°C, and cultivate for...

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Abstract

The invention relates to a vaccine for fish, in particular to a whole-cell vaccine prepared from Aeromonas caviae or a vaccine prepared from exotoxin of the Aeromonas caviae, and application thereof in preventing fish diseases. A strain is obtained through the separation from a focus of southern catfish, is the Aeromonas caviae through the identification, is named DKN-1, and was preserved in China Center for Type Culture Collection on Sep 12th, 2007, and a preserving number of the strain is CCTCC NO: M207146. The use of the vaccine can stimulate fish bodies to produce the immunity against ulcer syndrome, and strengthen the resistance against diseases.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to a whole-bacteria vaccine prepared from Aeromonas caviae or a vaccine prepared from its exotoxin, and its application in fish disease prevention and treatment. Background technique [0002] Bacteria of the genus Aeromonas (Aeromonas) widely exist in water and soil in nature, and most of them are saprophytic bacteria and conditional pathogenic bacteria, and are common residents of normal fish. When the water body is seriously polluted or the fish body is injured, these bacteria can cause the fish body to become ill or even die. The bacteria of the genus Aeromonas are divided into two categories: one is the psychrophilic Aeromonas group; the other is the mesophilic Aeromonas group. The former is represented by Aeromonas salmonicida (A.salmoida), which has no motility and is only pathogenic to some cold-water fish, such as rainbow trout and Atlantic salmon. species, Japanese salmon subspecies and ...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04A61P17/02
Inventor 肖丹汪开毓耿毅陈德芳刘建平刘匆刘海燕罗远会
Owner TONGWEI
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