Method for tissue culture, rapid propagation and cultivation of carnation seedling

Abstract

The present invention provides a rapid propagation culture method of carnation tissue culture seedling, and the method comprises the steps of cutting stock and sterilizing the explant, enrichment culturing and rooting culturing. Namely in the rooting period of seedling, the traditional method that the non-root stem segment is executed with in-bottle rooting is replaced. The non-root stem segment is directly inserted into a hybrid culture medium for primary rooting and seedling formation. The technical operation instruction of rooting culture technical system is simplified. The shearing of tissue cultured non-root seedling is executed in big shelter. A program of rooting culturing in bottle is saved. The material which is sheared for micro-sticking can include stem tip, stem segment and stem base. The material which can be used for rooting according to traditional in-bottle rooting culture program only includes stem tip. The rooting material is more than 3-4 times compared with the traditional method. The cultured rooting seedling can become finished seedling after outdoor transition management without secondary replanting. The produced seedlings has the advantages of strongness, orderliness and excellent quality under the precondition that the cost is saved. The standard of excellent-quality seeding is obtained. The rapid propagation culture method of carnation tissue culture seedling is suitable for the large-scale production of flower seedling.

Description

technical field [0001] The invention relates to a technology of rapid propagation of carnation tissue culture seedlings combined with rooting out of a bottle for one-time seedling formation, especially a large-scale production technology of carnation tissue culture seedlings. Background technique [0002] Carnation (Dianthus caryophyllus L), also known as carnation, is a perennial herbaceous perennial plant of the caryophyllaceae Caryophyllaceae. Native to Northern and Southern Europe. There are about 80 species of plants in this genus. They are native plants of British Ireland, Northern Europe and Southern Europe. They like a cool, dry, sunny and well-ventilated ecological environment. Carnation has good cold resistance, but poor heat resistance. The optimum growth temperature is 14-21°C. When the temperature exceeds 27°C or is lower than 14°C, the plant grows slowly. Suitable for planting in humus-rich, well-drained calcareous soil, likes fertilizer. [0003] Regarding ...

AI Technical Summary

Problems solved by technology

[0003] Regarding the tissue culture of Carnation, since the 1980s, researchers at home and abroad have carried out a large amount of research work on the explant parts, medium components, culture conditions, and preservation of detoxified seeds of Carnation, but these are limited. Regarding the research on a certain link in the tissue culture technology, although the research on the tissue culture technology and the large-scale production of carnation seedlings have also been reported in China, it has not systematically involved the one-time seedling growth of carnation by micro-tipping the outer stem section of the bottle. technology

[0004] The traditional tissue culture seedlings are rooted on the basis of the subculture bottle seedlings, and the rooting coefficient is not high. After rooting, they are moved to the perlite matrix for the first stage transition, and then transplanted to the mixed seedlings after they survive. The secondary transition is carried out in the substrate, and the seedlings cultivated at this time have thin and tall stems, so it is not easy to survive

Coupled with the low output of traditional tissue culture seedlings, complicated training procedures and high costs, it has been difficult to meet market demand