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Scuffing experimental device for cell migration research and high throughput medicament sifting motion

A technology for scratch experiment and cell migration, which is applied in biochemical cleaning equipment, enzymology/microbiology equipment, microbial measurement/inspection, etc. It can solve the problems of uncertain scratch position, inconvenient photo taking, and non-straight scratches, etc. , to reduce errors and achieve accurate and reliable results

Inactive Publication Date: 2009-08-19
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current scratch test, a sterile plastic tip is generally used for manual scratching, which has defects such as not straight scratches, uncertain scratch positions, inconvenient photography and subsequent quantitative statistical analysis.
In addition, the current scratch method is not suitable for high-throughput drug screening targeting cell migration

Method used

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  • Scuffing experimental device for cell migration research and high throughput medicament sifting motion
  • Scuffing experimental device for cell migration research and high throughput medicament sifting motion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] According to the needs of the experiment, select an appropriate number of rubber scratch bar strips and fix them at the corresponding positions with screws. Before the experiment, the mounted device was irradiated with ultraviolet light to sterilize it.

[0013] Spread the cells in the cell culture plate, remove the plate cover of the cell culture plate during the scratch test, put the present invention on the cell culture plate, fold down the side wall 1 and side wall 2, and stretch the side wall 3 and side wall 4 Open it so that the rubber scratch stick is located at the far left of the well of the cell culture plate, push it slightly to the right, and draw a scratch; then fold down sidewall 3 and sidewall 4, and stretch out sidewall 1 and sidewall 2. Place the rubber scratch stick on the uppermost edge of the well of the cell culture plate and push down gently to make a scratch. In this way, a criss-cross scratch is produced. Then, wash off the crossed-out cells an...

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Abstract

The invention relates to a simple and rapid scratching test device, which is applicable to the cell migration research and the high throughput drug screening taking the cell migration as a target spot. The scratching test device is provided with a demountable rubber scratching head, takes a cell culture plate containing a plurality of pores (6, 12, 24, 96, 384 pore plates and the like) as an experimental object, can be precisely sheathed on the cell culture plate and only needs to be lightly pushed to generate the neat and uniform scratch and the scratching position is fixed, thus being convenient for taking pictures and subsequent statistical analysis. The scratching test device is also applicable to an automation working platform of a high throughput screening system and used for rapid screening the medicine taking the cell migration as the target spot. In addition, the scratching test device can be repeatedly used and the rubber scratching head has the demountable function and can be used after being sprayed by alcohol and irradiated by ultraviolet. The invention provides a simple and rapid method for the scratching test, which has great market prospect.

Description

field of invention [0001] The invention relates to a simple and rapid scratch test device, which is suitable for cell migration research and high-throughput drug screening with cell migration as the target. Background of the invention [0002] Cell migration is ubiquitous in the normal physiological activities of the human body and in the occurrence of diseases, such as cell foraging, wound healing, embryogenesis, nervous system formation, immune response, cancer metastasis and many other events are involved in cell migration. Cell migration is an important direction and focus of current life science research. Scientists are trying to achieve greater results in medical applications such as vascular injury repair, prevention of cancer metastasis, and skin grafting through the study of cell migration. [0003] At present, there are two main experimental methods for studying cell migration: scratch experiment and transwell experiment. Epithelial cells, keratinocytes, etc. form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/02
Inventor 周军谢松波高金珉
Owner NANKAI UNIV
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