Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide and application of same

An antibacterial peptide and serum powder technology, which is applied to antiviral agents, digestive systems, medical raw materials derived from arthropods, etc., can solve the problems of no description of active E. Achieve rapid drying and sterilization ability, shorten drying time, and induce good effect

Active Publication Date: 2009-09-16
BIOPHARM RES & DEV CENT JINAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the above-mentioned technical problems, the patent application with the application number 03128069.2 discloses "a preparation method for extracting polypeptide antibiotics from silkworm bodies", but the disadvantage of this application is that the concentration of active E. Live Escherichia coli is bound to cause pollution, and it is impossible to induce antimicrobial peptides by eating Escherichia coli orally; in addition, the induction condition of the ultrasonic wave is 10-20 kHz for 2-5 hours, and the lowest frequency of the ultrasonic wave is above 20 kHz, 10 kHz He is not ultrasonic, and the power of ultrasonic is not specified. Ultrasonic can only be transmitted in water or other carriers. If the silkworm body is immersed in water for 2 to 5 hours, it will inevitably lead to suffocation and death; the activity of the antimicrobial peptide in the silkworm body before extraction Description, so the preparation method of this patent is not feasible

Method used

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  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide and application of same
  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide and application of same
  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Inducing the production of antimicrobial peptides and processing methods for silkworm cooked silkworms, comprising the following steps:

[0044] 1. Collection of cooked silkworms: Collect silkworm larvae at the end of the fifth instar of silkworms, remove residual mulberry and silkworm excrement, and remove diseased silkworms and immature silkworms as materials, taking 10Kg of materials as an example.

[0045]2. Spread the cooked silkworms on a plastic net, and spray it with 50°C warm water through a sprayer to achieve the cleaning effect.

[0046] 3. Put the washed cooked silkworms into a container of about 40Kg of normal temperature water, treat them with a square ultrasonic probe, ultrasonic frequency 30 kHz, power 300 watts, time 30 minutes, temperature 20 ℃, induce the production of antimicrobial peptides.

[0047] 4. Pick up the cooked silkworms after induction, dry them in the air, and place them in a sterile room at 25°C and a relative humidity of 80% to allow t...

Embodiment 2

[0081] The present invention induces production of antimicrobial peptides and processing methods thereof on tussah silkworm chrysalis, specifically comprising the following steps:

[0082] 1. Collection of tussah silkworm chrysalis: cut the tussah silkworm cocoons in autumn to obtain diapause pupae, and remove diseased and dead pupae. Use 100Kg fresh pupa as material.

[0083] 2. Ultrasound-induced antimicrobial peptides, spread silkworm chrysalis on a plastic net, spray and wash with warm water at 50°C, put it into a container of about 700 liters, and inject it with tap water at normal temperature. The ultrasonic generator was purchased from the square ultrasonic probe of Acason Company. Ultrasonic waves are generated after electrification, and an ultrasonic probe is placed on each of the four walls of the container to ensure that the ultrasonic waves spread evenly in the container. The ultrasonic frequency is 25 kHz, the power is 250 watts, and the continuous treatment is 1...

Embodiment 3

[0088] The processing method of tussah silkworm chrysalis serum dry powder of the present invention specifically comprises the following steps:

[0089] 1. Collection of tussah silkworm chrysalis: cut the cocoons of tussah silkworms in autumn to obtain diapause pupae, and remove sick pupae and dead pupae. With 100Kg fresh pupa as material.

[0090] 2. Ultrasound-induced antimicrobial peptides. Lay silkworm chrysalis on plastic nets, spray and wash them with warm water at 50°C, put them into a container of about 700 liters, and inject them with tap water at normal temperature. The ultrasonic generator was purchased from the square ultrasonic probe of Acason Company. Ultrasonic waves are generated after electrification, and an ultrasonic probe is placed on each of the four walls of the container to ensure that the ultrasonic waves spread evenly in the container. The ultrasonic frequency is 25 kHz, the power is 250 watts, and the continuous treatment is 25 minutes at a temperatu...

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Abstract

Pupa serum is made into dry powder, and the bactericidal activity of antibacterial peptide is kept for 300-3,000 unit / gram. Silkworm powder or pupa powder containing antibacterial peptide after oral taking can inhibit the multiplication of duck hepatitis B virus (DHBV) DNA in a body of duck, and the inhibition ratio reaches more than 80 percent. The silkworm powder, the pupa powder or pupa serum powder containing the antibacterial peptide is taken as a Chinese medicinal raw material and prepared into capsules for treating hepatitis.

Description

technical field [0001] The invention relates to the technical field of inducing silkworm or tussah silkworm chrysalis to produce antimicrobial peptide (cecropin); in particular, it relates to a preparation method and application of silkworm powder or chrysalis powder and chrysalis serum powder containing silkworm chrysalis antimicrobial peptide. Background technique [0002] According to the data of GenBank, more than 1200 kinds of insect and frog antimicrobial peptides have been published, including more than 50 kinds of silkworm and tussah silkworm antimicrobial peptides. Antimicrobial peptides induced from silkworm or tussah chrysalis can be directly used as antibacterial drugs. Usually, to induce silkworm or tussah silkworm chrysalis to produce antimicrobial peptides is to inject Escherichia coli or Enterobacter cloacae into the body, and produce antimicrobial peptides in vivo after a period of incubation. Because the injection of Escherichia coli will inevitably cause ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P1/16A61P31/20
Inventor 黄自然黄亚东苏志坚邓小娟项琪
Owner BIOPHARM RES & DEV CENT JINAN
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