Method for manufacturing potassiumtetroxalate through fermentation of Aspergillus niger

A technology of Aspergillus niger fermentation and potassium tetraoxalate, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high environmental pollution, high cost of potassium tetraoxalate, etc., and achieves the effect of reducing production costs

Inactive Publication Date: 2009-09-16
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0005] The technical problem to be solved by this invention is: aiming at the relatively high cost and high environmental pollution problems of chemical industry synthesis potassium tetraoxalate in the above-mentioned prior art, a kind of method for ...

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with embodiment.

[0011] First, isolate and purify Aspergillus niger: select the stem section of Huperus frugosa plant, wash it with running water for 2-3 hours → 75% alcohol disinfection for 1-2min → 0.01mol / L hydrochloric acid, 5% sodium hypochlorite, 2.4mmol / L lemon Rinse once with acid → 5% sodium hypochlorite disinfection for 10-12 minutes → rinse with sterile water for 3-5 times → disinfect with 10% hydrogen peroxide for 8-12 minutes → rinse with sterile water for 4-5 times → absorb surface water on sterile absorbent paper → inoculate On the PDA plate→separate and purify to obtain Aspergillus niger.

[0012] Example 1: Peel 300g of fresh potatoes, weigh 200g of potatoes with a size of 1cm cube, add 800mL of distilled water to boil for 30 minutes, filter, replenish the volume of the filtrate to 900mL, add 5g of sucrose, adjust the pH value to 5.3, and sterilize. Inoculate 10 mL of Aspergillus ni...

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Abstract

A method for manufacturing potassiumtetroxalate through fermentation of Aspergillus niger includes the steps of separation and purification of Aspergillus niger as well as the liquid fermentation culture of Aspergillus niger. Specifically, each liter of PDA fluid nutrient medium is added with 5-25g of sucrose and the pH value is adjusted to 4.7-6.4; in germ-free condition, the spore inoculum concentration of Aspergillus niger is 1 percent, the spore consistency is 1*10<4>-10<6>cfu/ml, the culture temperature is 30-40 DEG C, and constant-temperature fermentation culture proceeds for 72-100h; mycelia is filtered, the fermentation liquor is concentrated to one twentieth to one fifteenth of the total volume of culture solution; and finally white powered product of potassiumtetroxalate is obtained after separating out crystals, washing by ultrapure water and drying. By combining the separation and purification of Aspergillus niger with the liquid fermentation culture and using potato and sucrose as basic culture substrates which are safe and free of poison, the method realizes the full-energy environment-friendly production of potassiumtetroxalate during the whole process and reduced the production cost greatly.

Description

Technical field: [0001] The invention relates to a method for producing potassium tetraoxalate by fermenting Aspergillus niger, in particular to a method for producing potassium tetraoxalate by using separation and purification of Aspergillus niger and liquid fermentation culture of Aspergillus niger. Background technique: [0002] Potassium tetraoxalate, the chemical name is also called potassium trihydrogen oxalate, potassium trihydrogen oxalate, potassium tetraoxalate; the English name is potassium trihydrogen dioxalate dehydrate or potassium tetraoxalate; the molecular formula is KH 3 (C 2 o 4 ) 2 2H 2 O or C 4 h 3 KO 8 2H 2 O; molecular weight 254.19. The pure product is colorless crystal or white powder. When heated to 100°C, it loses water and becomes anhydrous, and decomposes into potassium carbonate and carbon monoxide at a higher temperature. It can be dissolved in water and slightly soluble in alcohol. [0003] The current market price of domestic analyt...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/685
Inventor 禹利君史云峰胡维新刘仲华范铁芳
Owner HUNAN AGRICULTURAL UNIV
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