Method for manufacturing potassiumtetroxalate through fermentation of Aspergillus niger

Abstract

A method for manufacturing potassiumtetroxalate through fermentation of Aspergillus niger includes the steps of separation and purification of Aspergillus niger as well as the liquid fermentation culture of Aspergillus niger. Specifically, each liter of PDA fluid nutrient medium is added with 5-25g of sucrose and the pH value is adjusted to 4.7-6.4; in germ-free condition, the spore inoculum concentration of Aspergillus niger is 1 percent, the spore consistency is 1*10<4>-10<6>cfu / ml, the culture temperature is 30-40 DEG C, and constant-temperature fermentation culture proceeds for 72-100h; mycelia is filtered, the fermentation liquor is concentrated to one twentieth to one fifteenth of the total volume of culture solution; and finally white powered product of potassiumtetroxalate is obtained after separating out crystals, washing by ultrapure water and drying. By combining the separation and purification of Aspergillus niger with the liquid fermentation culture and using potato and sucrose as basic culture substrates which are safe and free of poison, the method realizes the full-energy environment-friendly production of potassiumtetroxalate during the whole process and reduced the production cost greatly.

Description

Technical field: [0001] The invention relates to a method for producing potassium tetraoxalate by fermenting Aspergillus niger, in particular to a method for producing potassium tetraoxalate by using separation and purification of Aspergillus niger and liquid fermentation culture of Aspergillus niger. Background technique: [0002] Potassium tetraoxalate, the chemical name is also called potassium trihydrogen oxalate, potassium trihydrogen oxalate, potassium tetraoxalate; the English name is potassium trihydrogen dioxalate dehydrate or potassium tetraoxalate; the molecular formula is KH 3 (C 2 o 4 ) 2 2H 2 O or C 4 h 3 KO 8 2H 2 O; molecular weight 254.19. The pure product is colorless crystal or white powder. When heated to 100°C, it loses water and becomes anhydrous, and decomposes into potassium carbonate and carbon monoxide at a higher temperature. It can be dissolved in water and slightly soluble in alcohol. [0003] The current market price of domestic analyt...

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Problems solved by technology

[0005] The technical problem to be solved by this invention is: aiming at the relatively high cost and high environmental pollution problems of chemical industry synthesis potassium tetraoxalate in the above-mentioned prior art, a kind of method for Aspergillus niger fermentation production potassium tetraoxalate is provided, using potato, sucrose as The basic culture substrate is safe and non-toxic, and realizes the low-cost, all-round and environmentally friendly production of potassium tetraoxalate