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Recombinant human keratinocyte growth factor-2 containing eye drops and method for preparing same

A technology of keratinocytes and growth factors, applied in the field of recombinant human keratinocyte growth factor-2 eye drops and its preparation, can solve the problems of affecting eyesight, strong permeability, easy bleeding and secondary fibrosis, etc. The effect of stability and application convenience

Active Publication Date: 2009-09-23
广州力鑫生物科技有限公司
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  • Description
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AI Technical Summary

Problems solved by technology

The structure of corneal neovascularization is often fragile and highly permeable, prone to hemorrhage and secondary fibrosis, which seriously affects vision and even blindness

Method used

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  • Recombinant human keratinocyte growth factor-2 containing eye drops and method for preparing same
  • Recombinant human keratinocyte growth factor-2 containing eye drops and method for preparing same
  • Recombinant human keratinocyte growth factor-2 containing eye drops and method for preparing same

Examples

Experimental program
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Effect test

Embodiment Construction

[0010] 1. Prescription Examples

[0011] prescription:

[0012] KGF-2 100μg

[0013] Glycerin 2g

[0014] Methionine 0.2g

[0015] Histidine hydrochloride 1g

[0016] EDTA0.2g

[0017] Tween 80 0.01g

[0018] Sodium chloride 0.5g

[0019] Add 50mM phosphate buffer to 100ml

[0020] preparation:

[0021] Weigh the prescribed amount of glycerin, methionine, histidine hydrochloride, EDTA, Tween 80 and sodium chloride and dissolve in 60 ml of 50 mM phosphate buffer solution, and sterilize with flowing steam (115° C., 30 minutes). After cooling, add the prescribed amount of KGF-2 in a local 100-level environment, stir well, add 50mM phosphate buffer to about 90ml, adjust the pH to 7.0, and add 50mM phosphate buffer to 100ml. Filter through a 0.22μm filter membrane, and then pack.

[0022] Stability experiment:

[0023] According to "Chinese Pharmacopoeia" (2005 edition), the accelerated stability test was carried out. Temperature 25℃±2℃, relative humidity 60%RH±5%. At ...

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Abstract

The invention discloses recombinant human keratinocyte growth factor-2 containing eye drops, which are characterized by consisting of recombinant human keratinocyte growth factor-2 as well as an antioxidant, a chelator, a stabilizer, an osmotic pressure adjusting agent, a surfactant and a proper buffer system in a certain proportion. The invention also discloses a method for preparing the eye drops. Pharmacological tests show that eye drops can better promote the repair of damaged corneal epithelium.

Description

technical field [0001] The invention relates to a recombinant human keratinocyte growth factor-2 eye drop and a preparation method thereof, in particular to a recombinant human keratinocyte growth factor-2 eye drop suitable for treating corneal epithelial damage caused by various reasons and its preparation method. Background technique [0002] The human cornea is divided into 5 layers, the outermost layer of non-keratinized squamous epithelium about 50 microns thick is called corneal epithelium. The corneal epithelium is often exposed to harsh environments and is easily damaged by physical, chemical, microbial, parasite, immune and other factors, often leading to delayed or non-healing of the corneal epithelium, and serious complications such as corneal ulcers and perforations. The clinical treatment is quite difficult. In addition, rapid and complete corneal epithelial repair after ophthalmic surgery and trauma is also necessary to maintain normal vision. At present, th...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/08A61P27/02
Inventor 李校堃王晓杰田海山王会岩马吉胜黄志锋黄亚东肖健周鑫朱佳男项琪
Owner 广州力鑫生物科技有限公司
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