Method for producing gamma-aminobutyric acid by separated fermentation of enterococcus faecium

A technology of aminobutyric acid and Enterococcus faecium, which is applied in the biological field to achieve the effects of improving utilization rate, convenient operation and reducing production cost

Inactive Publication Date: 2009-09-23
HANSHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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However, there is no report on the production of γ-aminob...

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  • Method for producing gamma-aminobutyric acid by separated fermentation of enterococcus faecium

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Embodiment 1

[0043] The preserved strains of Enterococcus faecium were activated on MRS slope, inserted into MRS or TYG or improved PD seed medium, stirred and cultured at 30-37°C, 100-180rpm for 12-24h, and then inserted into MRS or 4-10% TYG or improved PD fermentation medium, at 30-37°C, under stirring at 100-180rpm, add 1mol / L NaOH to control the pH6.0-7.0 culture for 24h, then remove the pH control, continue to ferment for 12-18h, press 15-40g Add glutamic acid or sodium glutamate at a ratio of final concentration / L, and continue to ferment at 35-45°C, pH 3.0-5.0 for 24-96 hours, so as to obtain a fermentation with a γ-aminobutyric acid content of 10-20g / L mash.

Embodiment 2

[0045] Enterococcus faecium is used as the production strain, firstly use the MRS slope to activate, and then use the MRS liquid culture in the triangular flask to activate based on 30-37°C, 100rpm shaker culture for 12h, and then insert 4-10% of the inoculum into MRS or TYG or improved PD seed medium, at 30-37°C, 100-180rpm, stir culture for 12-24h, then insert 4-10% into MRS or TYG or improved PD fermentation medium, at 30-37°C, 100-180rpm Under stirring, add 1mol / L NaOH to control the pH6.0~7.0 and cultivate for 24h, then release the pH control, continue to ferment for 12~18h, add glutamic acid or sodium glutamate at a ratio of 15~40g / L final concentration, The pH value is controlled at 3.0-5.0 by feeding NaOH and HCl and the fermentation is continued at 35-45° C. for 24-96 hours, thereby obtaining fermented mash with a content of γ-aminobutyric acid of 10-20 g / L. After sterilized discharge, GABA can be extracted and refined.

Embodiment 3

[0047] Enterococcus faecium is used as the production strain, firstly use the MRS slope to activate, and then use the MRS liquid culture in the triangular flask to activate based on 30-37°C, 100rpm shaker culture for 12h, and then insert 4-10% of the inoculum into MRS or TYG or improved PD seed medium, at 30-37°C, 100-180rpm, stir culture for 12-24h, then insert 4-10% into MRS or TYG or improved PD fermentation medium, at 30-37°C, 100-180rpm Under stirring, add 1mol / L NaOH to control the pH6.0~7.0 and cultivate for 24~36h, add glutamic acid or sodium glutamate at a ratio of 15~40g / L final concentration, and control the pH value by feeding NaOH and HCl Continue to ferment at 3.0-5.0 at 35-45° C. for 24-96 hours to obtain fermented mash with γ-aminobutyric acid content of 10-20 g / L.

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Abstract

The invention provides a method for producing gamma-aminobutyric acid by separated fermentation of an enterococcus faecium, which is characterized in that the enterococcus faecium is taken as a strain, the pH value and temperature condition of deep fermentation is controlled by step, and glutamic acid or sodium glutamate is supplemented externally, thus producing the gamma-aminobutyric acid by fermentation, wherein the concentration of the gamma-aminobutyric acid in a fermenting mash reaches 10-20g/L. The method has the advantages of simplifying the fermentation process, having high yield and utilization ratio of the equipment, convenient operation, and the like.

Description

technical field [0001] The invention relates to a method for producing gamma-aminobutyric acid by using microorganisms, in particular to a method for producing gamma-aminobutyric acid by submerged fermentation of Enterococcus faecium, and belongs to the field of biotechnology. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA), also known as aminobutyric acid, is a natural amino acid that is not composed of proteins. It is a major inhibitory neurotransmitter in the mammalian central nervous system and has important physiological functions. , such as lowering blood pressure, diuresis, analgesia, improving brain function, enhancing brain vitality, promoting long-term memory, nourishing nerve cells, improving menopausal syndrome, etc. Long-term lack of GABA in the brain will lead to epilepsy, Parkinson and other diseases. At the same time, GABA is also related to brain aging, and its deficiency will lead to "deafness and blindness" in the elderly. I...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/01
Inventor 杨胜远李云韦锦
Owner HANSHAN NORMAL UNIV
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