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Major gene for porcine intramuscular fat deposition and molecular marker thereof

A technique of intramuscular fat and main-effect gene, which is applied in the field of main-effect gene and molecular markers of intramuscular fat deposition in pigs, can solve problems such as difficulty in significantly increasing intramuscular fat content, and achieve the effect of huge economic benefits.

Active Publication Date: 2009-09-30
北京众仕和生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the genetic complexity of the trait of intramuscular fat content, it is difficult to significantly increase the content of intramuscular fat by simply using methods such as progeny determination and individual performance measurement

Method used

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  • Major gene for porcine intramuscular fat deposition and molecular marker thereof
  • Major gene for porcine intramuscular fat deposition and molecular marker thereof
  • Major gene for porcine intramuscular fat deposition and molecular marker thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Sequence cloning and analysis of regulatory genes, main effect genes and molecular markers of pig intramuscular fat deposition

[0046] 1. Amplification primers:

[0047]

[0048]PCR amplification conditions: (1) 94°C, 4min; (2) 94°C, 30s; (3) 54.2°C, 30s; (4) 72°C, 40s; (5) Goto (2) 35cycles; 72°C, 8min .

[0049] 2. Sequence determination and analysis:

[0050] ①PCR product gel cutting, purification and recovery: 100 μL PCR product, 1% agarose gel electrophoresis, under the ultraviolet gel imager (Vilber Lourmat, Tex-20LM, France), cut the gel, use the gel recovery kit (Beijing Yingying Sunshine Biotechnology Co., Ltd., Beijing) recovered and purified.

[0051] ② Connect with T carrier, the reaction system is as follows:

[0052]

[0053] 16 ℃ water bath overnight, 16h.

[0054] ③Transformation: Use 100 μL of competent cells, add 10 μL of ligation product, ice-bath for 30 minutes, water bath at 42°C, heat shock for 90 seconds, ice-bath for 2-5 min...

Embodiment 2

[0057] Example 2 Detection experiment of intramuscular fat content in pigs of different genotypes:

[0058] The intramuscular fat content of the longissimus dorsi muscle of different genotypes of CLIC5A was measured in Rongchang pigs (fat pigs) and PIC pigs (lean pigs) populations of 60kg and 90kg respectively, and it was found that the genotype of the gene was correlated with intramuscular fat closely related.

[0059] Four genotypes of AA, AB, BB and BC were detected in Rongchang pigs, accounting for 8.3%, 33.3%, 50% and 8.3% of the population respectively, and AC and CC were not detected.

[0060] figure 1 Indicates the intramuscular fat content of different genotypes of 60kg Rongchang pig CLIC-5, among which the AA type has the highest intramuscular fat content, reaching 8.15%; followed by the AB type, with an intramuscular fat content of about 4.5%; and the BB type is the lowest, with an average of 2.9% %.

[0061] Among the 90kg Rongchang pigs, the intramuscular fat c...

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Abstract

The invention provides a major gene for porcine intramuscular fat deposition and a molecular marker thereof, and discloses application of the major gene in pig breeding and preparation of products relative to gene diagnosis of the porcine intramuscular fat deposition. Based on the major gene and the molecular marker, a genetic diagnostic technique with porcine intramuscular fat deposition characteristics is developed, and a core swine herd with intramuscular fat super deposition is established, thus pig breeding workers can breed pig breeds with high lean meat percentage and high intramuscular fat content in a short time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a major gene for fat deposition in pig muscle and a molecular marker thereof. Background technique [0002] With the improvement of people's living standards, consumers are paying more and more attention to meat quality. Local pig breeds in my country are famous for their good meat quality and are widely praised by consumers. However, due to their low lean meat percentage, slow growth rate and low feed remuneration, they are excluded from the mainstream breeds of pork production systems at home and abroad. In the past half a century, pig breeding in the world has pursued too much lean meat ratio, resulting in the dominant pig breeds in pork production being lean-meat pigs, and the intramuscular fat content is generally lower than 2%. Pork meat quality is highly valued by consumers many criticisms. [0003] Intramuscular fat refers to the fat deposited between muscle fibers or insid...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/11C12Q1/68
Inventor 尹靖东李德发马润林
Owner 北京众仕和生物技术有限公司
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