Method for extracting zein by microwave

A technology of zein and corn gluten powder, applied in the direction of plant zein and peptide preparation methods, chemical instruments and methods, etc., can solve the problems of low biological value, pollution of the environment, waste of resources, etc., and achieve improvement Purity, increase added value, effect of reducing impurities

Inactive Publication Date: 2009-10-21
GUANGDONG FOOD IND INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein content of this kind of corn gluten powder is about 60%, but due to the lack of essential amino acids such as lysine and tryptophan, its biological value is low. Currently, it is mostly sold as l

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 200 g of corn gluten powder, add 1200 milliliters of water and mix. Adjust the pH to 5.5±0.1, add 0.3% α-amylase, and react at 75° C. for 4 hours. Raise the temperature to 130°C to inactivate the enzyme, adjust the pH to 4.0±0.1 after the temperature drops to room temperature, add 0.4% glucoamylase, and react at 50°C for 2 hours. Heat up to 100°C to inactivate the enzyme, filter, add 1600 ml of 70% ethanol solution to the filter residue, and extract by microwave for 10 minutes with a microwave power of 200 watts. Centrifuge to obtain the zein extract, add 4800 ml of water to dilute the ethanol concentration of the zein extract, let it stand for 5 hours to obtain the zein precipitate, and dry it with air at 80°C for 12 hours to obtain the zein product , the yield is 21.3%, and the protein content is 91.2%.

Embodiment 2

[0028] Weigh 150 g of corn gluten powder, add 600 milliliters of water and mix. Adjust the pH to 6.0±0.1, add 0.2% α-amylase, and react at 90° C. for 2 hours. Raise the temperature to 130°C to inactivate the enzyme, adjust the pH to 4.5±0.1 after the temperature drops to room temperature, add 0.6% glucoamylase, and react at 45°C for 2 hours. Heat up to 100°C to inactivate the enzyme, filter, add 1500 ml of 85% ethanol solution to the filter residue, and extract by microwave for 8 minutes with a microwave power of 300 watts. Centrifuge to obtain the zein extract, add 6000 ml of water to dilute the ethanol concentration of the zein extract, let it stand for 3 hours to obtain the zein precipitate, and dry it at 100°C for 6 hours to obtain the zein product , the yield is 23.8%, and the protein content is 92.4%.

Embodiment 3

[0030] Weigh 150 g of corn gluten powder, add 1200 milliliters of water and mix. Adjust the pH to 6.2±0.1, add 0.2% α-amylase, and react at 75° C. for 2.5 hours. Raise the temperature to 140°C to inactivate the enzyme, adjust the pH to 4.8±0.1 after the temperature drops to room temperature, add 0.7% glucoamylase, and react at 45°C for 2.5 hours. Heat up to 110°C to inactivate the enzyme, filter, add 1200 ml of 90% ethanol solution to the filter residue, and extract by microwave for 3 minutes with a microwave power of 500 watts. Centrifuge to obtain a zein extract, add 6000 ml of water to dilute the ethanol concentration of the zein extract, let stand for 10 hours to obtain a zein precipitate, and vacuum dry at 80° C. for 6 hours to obtain a zein product. The yield is 23.1%, and the protein content is 89.7%.

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Abstract

The invention discloses a method for extracting zein by microwave. The method comprises the following steps: corn protein powder is used as a raw material; complex enzyme is added step by step into the powder for treatment, and the obtained power is filtered; the filter residue is added into ethanol aqueous solution to be subjected to microwave extraction, and is centrifugally separated to obtain zein extract; water is added into the zein extract to dilute the ethanol concentration of the zein extract to obtain zein precipitate; finally, the zein precipitate is dried to obtain a zein finished product. The method adopts double enzymolys method to carry out pretreatment and microwave technology to carry out extraction, has the characteristics of high yield, high purity, low production cost and short production period, significantly improves the additional value of the corn protein powder, and contributes to the comprehensive development and utilization of the corn protein powder.

Description

technical field [0001] The invention relates to a method for extracting zein. Background technique [0002] Corn gluten meal, also known as corn gluten, is a by-product of the production of starch from corn. The protein content of this kind of corn gluten powder is about 60%, but due to the lack of essential amino acids such as lysine and tryptophan, its biological value is low. Currently, it is mostly sold as low-value feed protein, and many factories do not even make any use of it. , Let it be discharged with the waste liquid, which not only causes a waste of resources, but also seriously pollutes the environment. [0003] The zein protein involved in the present invention is the main protein in corn gluten meal. It is insoluble in water and absolute alcohol, soluble in 60-95% alcohol aqueous solution, and also soluble in strong alkali, acetone, acetic acid and other organic solvents. The zein solution has film-forming properties. When the solvent evaporates, the protei...

Claims

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Application Information

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IPC IPC(8): C07K14/425C07K1/14
Inventor 梁丽敏徐勇王欣骆海平
Owner GUANGDONG FOOD IND INST
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