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In-situ inoculating operation method for cerebral tumor tissue block animal and inoculating needle thereof

A technology of tumor tissue and tissue blocks, which is applied in surgery, puncture needles, puncture needles, etc., can solve the problems of loss of structural characteristics, large trauma, loss of parentage, etc., to ensure the success rate of transplantation, short operation time, and high degree of simulation Effect

Inactive Publication Date: 2009-11-04
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cells inoculated by the first method will inevitably mutate due to long-term growth in vitro, lose their parental nature, and the simulation is not satisfactory; The number of living cells injected into the brain is guaranteed, so the success rate of inoculation is difficult to guarantee. At the same time, because the original tumor tissue structure loses its structural characteristics after being digested, the simulation is insufficient; the third method can realize animal tumor models However, since opening the skull is a very traumatic operation, the postoperative mortality rate of animals is very high, which limits its application.

Method used

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  • In-situ inoculating operation method for cerebral tumor tissue block animal and inoculating needle thereof
  • In-situ inoculating operation method for cerebral tumor tissue block animal and inoculating needle thereof
  • In-situ inoculating operation method for cerebral tumor tissue block animal and inoculating needle thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0044] 1. Required instruments and materials: miniature cranial drill, tissue block injection needle, tissue pusher, medical forceps or small medicine spoon, plate, small medical scissors.

[0045] 2. Surgical steps

[0046] (a) Take the brain tumor specimen and cut it into pieces with small medical scissors;

[0047] (b) Put the chopped tumor tissue 1 into the funnel of the tissue block injection needle 2 with a medicine spoon ( figure 1 ); use the pusher 3 to squeeze the tissue block injection needle 2, so that the tumor tissue fragments 1 are tightly squeezed into the needle tube of the injection needle 2 ( figure 2 ), excess tumor tissue fragments 1 overflowed from the front end of the needle tube ( image 3 ); insert the needle core 4 in the needle tube, and continue to push the tumor tissue particles 1 in the needle tube with the needle core 4 ( Figure 4 ), and then quantify the volume of tumor tissue particles 1 left in the needle tube according to the scale marked...

experiment example

[0058]The brain tumor tissue was cut into small pieces for brain transplantation in nude mice. Experimental nude mice were anesthetized by intraperitoneal injection of 10% chloral hydrate 200 mg / kg, placed in the abdominal recumbent position, and their limbs and tail ends were fixed on plastic plates. Disinfect the skin of the head with povidone iodine, cut the skin 0.5cm in the middle of the top of the mouse head, and expose the skull. Drill the skull 1.0mm in front of the anterior cranium and 2.5mm to the right of the midline with a 1.0mm diameter miniature cranial drill 6 to the dura mater 8 . Through the tissue pusher 3, push the above-mentioned tumor tissue fragments 1 into the tissue block injection needle 2, and adjust the total volume of the tumor fragments to 2.0mm 3 . Then insert the tissue block injection needle 2 with a controllable depth vertically and slowly into the mouse brain 4.0 mm by hand, retract about 1 mm, slowly push the tumor tissue fragments 1 in, st...

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Abstract

The invention discloses an in-situ inoculating operation method for a cerebral tumor tissue block animal. The method comprises the following steps: (a) taking a cerebral tumor tissue, and shearing the cerebral tumor tissue into tumor tissue particles; (b) placing the tumor tissue particles into a needle tube of a tissue block injection needle, and carrying out quantifiable indwelling control on the tumor tissue particles; (c) anaesthetizing the animal, dissecting the skin on the surface of the animal skull, and boring a hole on the animal cranial bone; (d) inserting the tissue block injection needle along a cranial bone hole, and slowly injecting the tumor tissue particles; and (e) slowly pulling out the needle, sealing the cranial bone hole by bone wax, and suturing the scalp. The method can ensure the original tissue structural property of an inoculated tumor tissue, provides a tumor model with high simulation degree for tumor research, can ensure the vitality of inoculated cells, has high tumor transplantation success rate, can realize quantification of cell number in the inoculated tumor tissue and positioning of inoculation in the tumor tissue animal brain, has short operation time, and can meet the requirement of needing large amount of animal models in once experiment.

Description

technical field [0001] The invention relates to the field of brain tumor model establishment in medical research, in particular to a brain tumor tissue block animal in situ inoculation operation method, and a brain tumor tissue block animal in situ inoculation needle used in the method. Background technique [0002] In medical research, especially in the study of brain tumors, it is very necessary to establish qualified animal models of brain tumors for further scientific research. At present, scientists in the field of brain tumor research around the world mainly use three methods to establish corresponding animal models: [0003] (1) The brain tumor cells that have been subcultured in vitro for a long time are injected into the brains of animals under the assistance of a stereotaxic instrument; [0004] (2) Fresh brain tumor tissue blocks are chemically digested into a single-cell suspension and then injected into the animal brain; [0005] (3) After the animal's skull i...

Claims

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Application Information

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IPC IPC(8): A61D1/02
Inventor 黄强李如军刁艺赵耀东董军兰青
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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