New preparation technology and new applications of syringa amurensis rupr bar extract components
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A technology of extracting and extracting Martini, which is applied in botany equipment and methods, medical preparations containing active ingredients, chemicals for biological control, etc., can solve the problem of anti-inhibiting components of unexploded Martini bark extract Bacteria research and other issues
Inactive Publication Date: 2009-11-11
JILIN AGRICULTURAL UNIV
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[0003] At present, there are few reports on the chemical constituents and pharmacological effects of the bark of Cinnamomum paliscens. There are only a few reports on the isolation of several compounds from the bark and branches, and no antibacterial components of the bark extract of C. Research
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Embodiment 1
[0019] (1) Take 5kg of the bark of Cinnamon chinensis, pick out the sundries, wash with water, dry or dry in the air, pulverize, extract with 8 times the amount of 10% ethanol at room temperature for 10h, and then ultrasonic for 1h, the temperature is 30°C and the power is 80W, then filter once with silk cloth and filter paper to obtain the filtrate;
[0020] (2) The filter residue of the bark of Cinnamomum chinensis after (1) treatment repeats (1) step each once with 8 times of amount and 6 times of amount of 10% ethanol respectively, obtains filtrate respectively, merges three times of filtrate;
[0021] (3) The above-mentioned (2) filtrate is reclaimed ethanol to obtain the bark extract of Cinnamon chinensis, and the yield is 23%.
[0022] (4) the above-mentioned (3) bark extract of Pamarinosa bark, upper D 101 Too porous resin column, with H 2 O is eluted successively with 10%, 30%, 50%, and 70% ethanol after removing impurities to obtain macroporous resin ethanol elutio...
Embodiment 2
[0025] (1) Take 10kg of the bark of Cinnamon chinensis, pick out the sundries, wash with water, dry or dry in the air, pulverize, extract with 10 times the amount of 10% ethanol at room temperature for 12h, then ultrasonic for 1h, the temperature is 30°C and the power is 80W, then filter once with silk cloth and filter paper to obtain the filtrate;
[0026] (2) The filter residue after the treatment of the bark of Cinnamon chinensis through (1) repeats the steps of (1) once with 8 times of amount and 6 times of amount of 10% ethanol respectively, obtains the filtrate respectively, and merges the filtrate three times;
[0027] (3) Recover ethanol from the filtrate of (2) above to obtain the bark extract of Cinnamomum officinalis with a yield of 26%.
[0028] (4) the above-mentioned (3) bark extract of Pamarinosa bark, upper D 101 Macroporous resin column, with H 2 O is eluted successively with 10%, 30%, 50%, and 70% ethanol after removing impurities to obtain macroporous resi...
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Abstract
The invention relates to a new preparation technology and new applications of syringa amurensis rupr bark extract components, belonging to the technical field of research and development on medical products. The preparation technology comprises the steps of: drying and cutting up syringa amurensis rupr bark, leaching the syringa amurensis rupr bark in 10% ethanol with quantity being 8-10 times that of the bark for 10-12 hours at room temperature, conducting ultrasonic treatment for 1 hour at temperature of 30 DEG C and with power of 80W; using silk and filter paper for filtering one time separately, separately using ethanol with quantity respectively being 8 times and 6 times that of the filter residue to repeat above steps for once separately on filter residue, merging filtrate for three times, and recycling the ethanol to obtain syringa amurensis rupr crude extract; using H2O for removing impurities of D101 macroporous resin column on the crude extract, sequentially using 10%, 30 %, 50%, and 70% ethanol for elution to obtain macroporous resin ethanol elution components including (A) 10% ethanol, (B) 30% ethanol, (C) 50% ethanol and (D) 70% ethanol; and using H2O for removing impurities of JTY reversed-phase resin column on the macroporous resin ethanol elution10% component, sequentially using 2%, 5% and 10% ethanol for elution to obtain JTY reversed-phase ethanol elution components including (A-1) 2% ethanol, (A-2) 5% ethanol, and (A-3) 10% ethanol. The syringa amurensis rupr bark extract components have inhibitory actions with different degrees on colon bacillus, staphylococcus aureus, pasteurella, streptococcus and salmonella, therefore the extract components can be developed into natural antibacterial agents used for the industries of food, medicament, and the like, and prepare various products with antibacterial effects.
Description
technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The invention relates to a new preparation process and a new application of the bark extract components of Syringa amurensis Rupr. Background technique [0002] Syringa amurensis Rupr. is one of the common medicinal plants in Changbai Mountains. In my country, it is mainly distributed in Jilin, Liaoning, North China, Northwest China and Central China, with rich resources. The bark, trunk and branches can be used medicinally. It tastes bitter and slightly cold in nature. Modern pharmacological experiments have proved that it has the functions of clearing the lungs and eliminating phlegm, relieving cough, relieving asthma, reducing inflammation and diuresis. [0003] At present, there are few reports on the chemical constituents and pharmacological effects of the bark of Cinnamomum paliscens. There are only a few reports on the isolation of several compo...
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