Two-step method for cultivating microalgae by using light conversion film

A technology of light conversion film and microalgae, which is applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., to achieve the effects of fast transformation, improved transformation speed and product accumulation, and shortened transformation cycle.

Active Publication Date: 2011-07-06
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of light-converting film for the construction of microalgae cultivation greenhouses has not been reported, especially the use of light-converting film to cultivate microalgae at high density in two steps and induce the synthesis of useful products such as high value-added pigments.

Method used

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  • Two-step method for cultivating microalgae by using light conversion film
  • Two-step method for cultivating microalgae by using light conversion film
  • Two-step method for cultivating microalgae by using light conversion film

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Cultivation of Chlorella in two steps with light-converting film

[0031] Taking Chlorella (a kind of green algae) as an object, the first and second light conversion films are covered on the outer wall of the photoreactor to carry out two-step culture.

[0032] The first light conversion film (purchased from Jiangsu Mikeduo Company, the base material of the light conversion film is polyethylene SH150, the general formula of the light conversion agent is UV>RI-MS, the excitation spectrum peaks are at 300nm, 350nm and 550nm, and the emission spectrum peaks are at 650nm, the mass content of the light conversion agent in the base material is about 1.0%, and the thickness of the film is 0.10-0.16mm) covering the outer wall of the 10L bubble reactor, and the same reactor without the light conversion film is used as a control. Improved SM medium (medium composition and its content see Table 1-6 below), inoculate logarithmic algal species (formed 5-7 days after tr...

Embodiment 2

[0051] Embodiment 2: Two-step method of light conversion membrane to cultivate new green algae

[0052] As in Example 1, the cultured algae were Neochloris oleoabundans (purchased from UTEX), using BBM medium (reference: Stein, J. (ED.) Handbook of Phycological methods. Culture methods and growth measurements. CambridgeUniversity Press. 448 pages). The first light conversion film used mainly converts ultraviolet light into red light. It is purchased from Jiangsu Mikoduo Company. The base material of the light conversion film is polyethylene SH150. The general formula of the light conversion agent is UV>RI-MS, and the excitation spectrum peak At 300nm, 350nm and 550nm, the peak of the emission spectrum is at 650nm, the mass content of the light conversion agent in the base material is about 1.0%, and the thickness of the film is 0.10-0.16mm; the second light conversion film mainly converts ultraviolet light into blue light, the manufacturer As above, the base material of the...

Embodiment 3

[0053] Embodiment 3: Use the two-stage sunshine greenhouse covered with the first and second light conversion film to carry out two-step method to cultivate Chlorella

[0054] The culture objects are the same as those in Example 1, both being Chlorella. The general formula of the first-level greenhouse is UV>RI-MS. The mass content of the light-changing agent in the base material is about 1.0%, and the thickness is 0.10-0.16mm. The base material is polyethylene SH150, the excitation spectrum peak of the light transfer agent is at 300nm, 350nm and 550nm, and the emission spectrum peak is at 650nm). Curtains to adjust the excessive light at noon. There is a water curtain cooling system inside the greenhouse, and the reactor used inside the greenhouse is a glass tube bubbling reactor, and the culture system is 10m 3 , culture Chlorella with SM medium, inoculate the initial OD750 at 0.40, control the light intensity with sunshade curtains and reflective curtains, from the init...

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Abstract

The invention provides a two-step method for cultivating microalgae by using a light conversion film, comprising the following steps of: step one, carrying out the cultivation by using a first light conversion film so as to quickly accumulate the biomass; and step two, cultivating the microalgae obtained in the first step by using a second light conversion film, thus realizing the conversion of useful products, wherein the first light conversion film converts the light from the light source into the light with the wavelength within 400-700nm; and the second light conversion film converts the light from the light source into the light with the wavelength within 400-500nm.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation. The invention is an improvement of the conventional microalgae cultivation method, specifically, the invention provides a two-step method for cultivating microalgae by using a light conversion film. Background technique [0002] At present, the main methods of microalgae cultivation are open large pond cultivation (raceway pond) and closed photobioreactor cultivation. Open large pond culture is the earliest and simplest microalgae culture system, suitable for the cultivation of microalgae with relatively extensive growth conditions, such as spirulina, chlorella or Dunaliella salina. Its structure is simple and it mainly uses the environment Light and temperature conditions are used for cultivation, and the operation is simple and the cost is low. However, the microalgae culture is limited by environmental conditions. When the light intensity is low, the growth rate of the microalgae is slo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王慧岭马欣欣刘敏胜
Owner ENN SCI & TECH DEV
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