Method for washing and recycling membranate glass slide for microdissection

A technology for microdissection and glass slides, which is applied in the direction of cleaning methods using tools, cleaning methods and utensils, cleaning methods using liquids, etc. The effect of scientific research funds, low price and quantity saving

Active Publication Date: 2011-06-15
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these measures can achieve the purpose of completely removing residues while maintaining the adhesive properties and integrity of the film.

Method used

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  • Method for washing and recycling membranate glass slide for microdissection
  • Method for washing and recycling membranate glass slide for microdissection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Use this method to clean microdissected membrane slides of residual lung cancer tissue (paraffin-embedded specimen).

[0031] Take two microdissected membrane glass slides pasted with lung cancer tissue (paraffin-embedded specimen), one of which is not cleaned by this method, and one is cleaned by this method.

[0032] Place the microdissected membrane slides in xylene and incubate in a 50°C incubator for 20 minutes (this step is not required for frozen tissue specimens); place the slides in boric acid, alcohol, EDTA mixed solution A (mixed solution A is a mixed solution of 1% boric acid, 70% alcohol, and 0.25mol / L EDTA, and its volume ratio is 1:98:1) Soak in, shake for 4 minutes, and repeat once; rinse with double distilled water for 2 minutes; Transfer slides to pH 6, 80μg / ml proteinase K solution, incubate at 56°C for 2 hours; place slides in clean water, lightly touch the specimens on the membrane with fingers with plastic gloves, rinse with double distilled water ...

Embodiment 2

[0034] The cleaning and reuse method of the microdissection membrane glass slide can be implemented sequentially according to the following steps:

[0035] (1) Place the microdissected membrane slide in xylene and incubate in an incubator at 45-50°C for 20-30 minutes;

[0036] (2) Place the membranous slide in boric acid, alcohol and EDTA mixed solution A to soak, shake for 3 minutes, and repeat once; the concentration of the boric acid is 1%, the concentration of alcohol is 70%, and the concentration of EDTA is 0.25mol / L; the volume ratio of boric acid, alcohol and EDTA is 0.5:96.5:0.5.

[0037] (3) Rinse with double-distilled water for 3 minutes; transfer the membrane slide to proteinase K solution, and incubate at 56° C. for 1 hour; the pH of the proteinase K solution is 6, and the concentration is 90 μg / ml.

[0038] (4) Place the glass slide in clear water, lightly touch the specimen on the membrane in the membrane glass slide with fingers with plastic gloves, rinse with ...

Embodiment 3

[0042] The cleaning and reuse method of the microdissection membrane glass slide can be implemented sequentially according to the following steps:

[0043] (1) Soak the membrane glass slide in boric acid, alcohol and EDTA mixed solution A, shake for 5 minutes, and repeat once; the concentration of the boric acid is 1%; the concentration of the alcohol is 70%, the concentration of the alcohol is 70%. The concentration of EDTA is 0.25mol / L, and the volume ratio of boric acid, alcohol and EDTA is 1:97:1.

[0044] (2) Rinse with double distilled water for 3 minutes; transfer the membrane slide to proteinase K solution, and incubate at 56° C. for 2 hours; the pH of the proteinase K solution in the step (2) is 7, and the concentration is 100 μg / ml.

[0045] (3) Place the glass slide in clear water, lightly touch the specimen on the film in the membrane glass slide with fingers with plastic gloves, rinse with double distilled water, and remove large specimens;

[0046](4) Soak the g...

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Abstract

The invention discloses technology for washing and recycling a membranate glass slide for microdissection. The technology can be implemented according to the following steps: (1) dipping and shaking the membranate glass slide in mixed solution A of boric acid, alcohol and EDTA; (2) washing the membranate by using double distilled water, and transferring the membranate glass slide into prolease K solution to incubate; (3) placing the glass slide into clean water; slightly touching a sample on the membrane by a finger; and washing the membranate glass slide by the double distilled water; (4) dipping the glass slide in mixed solution B of hydrochloric acid, the alcohol and the EDTA; washing the membranate glass slide by using the double distilled water and absolute ethyl alcohol; and finishing the last washing in the absolute ethyl alcohol; and (5) quickly transferring the membranate glass slide onto a super-clean bench; quickly drying the absolute ethyl alcohol; and carrying out ultraviolet radiation at the same time. The technology removes tissues, nucleic acid, albumen and other micromolecular substances remained on the surface of a consumptive material, and can sufficiently maintain the completeness and adhering effect of the membranate glass slide for microdissection.

Description

technical field [0001] The invention relates to the field of cleaning microdissection molecular biology slices, in particular to a cleaning and reuse technology for microdissection membrane glass slides. Background technique [0002] The emergence of laser capture microdissection (Laser Capture Microdissection) technology and its system enables relevant scientific researchers to accurately separate the target tissue samples, cells (in vitro cultured living cells and tissue cells fixed in slices), organelles, etc. Even chromosomal bands are used for follow-up research, and the accuracy of the control samples is favored by scientific researchers all over the world. At present, most powerful research institutions have been equipped with this technology and related facilities. [0003] When tissue sections are used for microdissection, a special membrane glass slide consumable is required, which is expensive. At the same time, due to the need to enrich enough samples for the pur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/34B08B7/04B08B3/08B08B3/00B08B3/04B08B1/00B08B3/02C11D1/04C11D3/43
Inventor 高兴华齐瑞群陈洪铎
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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