Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm

A technology of chitosan and septicemia, which is applied in the application field of chitosan in the preparation of medicines for preventing and treating bacterial septicemia in silkworms, can solve the problems of inconspicuous, high cost, time-consuming and laborious bacteria control, and reduce the Infection risk, low cost, significant therapeutic effect

Inactive Publication Date: 2011-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current prevention and control methods mainly include sprinkling in vitro disinfectant powder on the silkworm body and killing the bacteria invading the body by adding antibacterial drugs. It is easy to induce drug resistance in silkworms, and the cost is relatively high
In addition, since silkworm chrysalis has become a delicacy on the dining table, after the silkworm chrysalis obtained from silkworms treated with these chemical agents are eaten by people, the residual chemicals will have adverse effects on human health.

Method used

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  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm
  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm
  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Dissolve chitosan powder with a deacetylation degree greater than 85% in 1% glacial acetic acid by volume, add distilled water to prepare chitosan solutions with concentrations of 0.01, 0.05 and 0.10 mg / mL, and take various concentrations of chitosan Chitosan solution 5mL, add the Bleeding Bacteria septicemia suspension of same concentration in every portion, make the concentration of bacteria in the mixed solution consistent (1.0×10 7 CFU / mL); use distilled water instead of chitosan powder as a contrast, check the number of germs after shaking at room temperature for 2 hours, the results are as follows:

[0015] The influence of table 1 different concentration chitosan solution on the growth of bleeding bacteria pathogen

[0016]

[0017] As can be seen from Table 1, under in vitro conditions, chitosan solutions of 0.01 mg / mL and 0.05 mg / mL can completely inhibit the proliferation of pathogenic bacteria, and chitosan solutions of 0.10 mg / mL can even kill existing ba...

Embodiment 2

[0019] Take the chitosan solution whose concentration is 0.10mg / mL, add a certain concentration of bleeding bacteria septicemia suspension, so that the concentration of bacteria in the solution is about 10 7 CFU / mL (7.00log 10 CFU / mL), the mixed solution was sampled and counted at 0.5, 1.0, 2.0 and 4.0 hours after mixing, and the number of bleeding and septicemia bacteria therein was recorded. Chitosan was replaced by distilled water as a control. The results showed that after chitosan was added to the germ suspension, the number of germs decreased sharply, showing a better bactericidal effect.

[0020] The influence of table 2 different culture time on chitosan solution (0.10mg / mL) antibacterial activity

[0021]

Embodiment 3

[0023] Soak mulberry leaves with a chitosan solution with a concentration of 0.10 mg / mL, feed the silkworms for 24 hours, inoculate the silkworms with Bleeding bacteria and septicemia, and use the following five treatments as controls: mulberry leaves were fed to silkworms, and silkworms were inoculated with pathogenic bacteria; ② mulberry leaves that had not been treated with chitosan were fed to silkworms, and silkworms were inoculated with distilled water; ③ Silkworms were fed with mulberry leaves treated with chitosan, and silkworms were inoculated with distilled water; ④ The silkworms were inoculated with distilled water; The mulberry leaves treated with chitosan were fed to silkworms, and silkworms were not inoculated; ⑤ The silkworms were fed with mulberry leaves treated with chitosan, and silkworms were not inoculated. Mortality of silkworms were counted at 24, 48 and 72 hours after inoculation.

[0024] Table 3 Chitosan solution treatment of mulberry leaf control effe...

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Abstract

The invention discloses an application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm. The drug can obviously reduce the risk of infection of the bacterial septicemia of silkworm and has obvious curative effect. The adoption of the drug for preventing and curing bacterial septicemia of silkworm is easy to operate and has lower cost; and more importantly,the preventing and curing links can not generate any threat to the health of workers working for preventing and curing.

Description

technical field [0001] The invention relates to the technical field of medicines for preventing and treating silkworm diseases, in particular to the application of chitosan in the preparation of medicines for preventing and treating silkworm bacterial septicemia. Background technique [0002] Sericulture is a traditional industry with resource advantages in China. It has a history of more than 5,000 years. As a traditional sideline of farmers, it is one of the important ways for farmers to increase their income. Bombyx mori is the economical insect with the largest breeding scale and the most mature technology. Due to its own characteristics, it has limited resistance to pathogenic bacteria and is very easy to be infected by bacteria and cause death. Bacterial septicemia caused by bleeding fungus (Serratia marcescens) is a common disease in sericulture production, which has a great impact on the high and stable yield of silkworm cocoons. [0003] The current prevention and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/722A61P7/00A61P31/04
Inventor 李斌余山红苏婷谢关林
Owner ZHEJIANG UNIV
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