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Flounder tetraplont fry batch induction method

A tetraploid, batch technology, applied in biochemical equipment and methods, fish farming, climate change adaptation, etc., can solve problems such as low triploid induction efficiency, inability to establish breeding colonies, and difficulty in large-scale promotion and application. , to achieve the effect of neat ploidy, strong practicability and high induction efficiency

Inactive Publication Date: 2009-12-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the disadvantages of low fish triploid induction efficiency, difficulty in large-scale popularization and application, only contemporary use, and inability to establish breeding colonies, and to establish a method to induce a large number of triploids by inhibiting the first cleavage of fish fertilized eggs. Method for tetraploid fry

Method used

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  • Flounder tetraplont fry batch induction method
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Embodiment Construction

[0030] Using modern biotechnology means, research polyploid induction technology of flounder fish, develop tetraploid fish, and then mate with diploid fish to produce sterile triploid fish population, so as to mass produce sterile fish group.

[0031] Homologous sperm are used to fertilize flounder eggs, and the fertilized eggs are subjected to hydrostatic shock treatment for a certain period of time after fertilization to inhibit the first cleavage and double the chromosomes, which can successfully induce the production of tetraploid fry of flounder, and establish The high-efficiency induction technology for tetraploid fry commonly used in marine flounder and flounder is used for sex control, sterile triploid seed cultivation and sterile seed breeding of these flounder and flounder fish, so as to improve the breeding output and product quality of these fish And economic benefits, has important practical significance and huge potential for promotion and application.

[0032] ...

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Abstract

The invention discloses a flounder tetraplont fry batch induction method comprising an induction method and an identification method of tetraplont fries; wherein, the induction method comprises collection and insemination of unfertilized ovum, and determination of oosperm chromosome doubling starting time, hydrostatic pressure shock pressure and processing time, and the identification method comprises ploidy determinator identification and chromosome analysis identification. The method firstly builds a method of inducing the tetraplont fries of flounder, such as paralichthysolivaceus and the like by hydrostatic pressure, screens hydrostatic pressure processing starting time and hydrostatic pressure shock conditions for inhibiting first merogenesis and carrying out chromosome doubling, and builds a tetraplont fry batch induction method. Tetraplont fries obtained with the method have high induction rate of 90%; the method of the invention has the characteristics of advancing, high efficiency, accuracy and reliability, and has important application value in fish polyploidy induction and sterile offspring seed production, and has wide promotion and application prospect in fish cultivation and breeding.

Description

Technical field: [0001] The invention belongs to aquatic genetic breeding technology, and relates to a method for inducing tetraploid of flounder and flounder by physical means. Background technique: [0002] Flounder, turbot and star flounder are important marine fishes in my country. At present, the annual output value of flounder and flounder in my country is close to 10 billion yuan. However, because many flounder and flounder are sexually mature relatively early, and the gonad development affects the growth and meat quality of the fish and reduces the quality of the product, research on the sterile technology of these fish has been carried out, and sterile populations have been developed, which is very important for improving the quality of these fish. The breeding output and commodity value of species are very important. In addition, more and more types of marine flounder and flounder are being introduced into my country, for example, desert flounder and Atlantic flou...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 陈松林王磊季相山田永胜邵长伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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