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Method for preparing micro-encapsulation bifidobacteria

A bifidobacterium microencapsulation technology, applied in the field of bifidobacterium microcapsule preparations and bifidobacteria preparations

Inactive Publication Date: 2009-12-09
任发政
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention is the first in the field of microcapsule technology at home and abroad, whether it is the selection of wall materials or the way of gelling.

Method used

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  • Method for preparing micro-encapsulation bifidobacteria
  • Method for preparing micro-encapsulation bifidobacteria
  • Method for preparing micro-encapsulation bifidobacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1-9

[0040] (1) The preserved Bifidobacterium longum BBMN68 (preserved in China Agricultural University) was subcultured twice to rejuvenate, and the strain morphology of the culture solution was examined under a microscope, and the activated bacterial strain was divided into 10 7 cfu / mL was inoculated in MRS medium and cultured anaerobically for 24 hours.

[0041] (2) Centrifuge the bacterial liquid obtained in (1) at 4000 rpm for 10 minutes to obtain bacterial cells, and collect the bacterial sludge.

[0042] (3) Prepare a certain concentration of skim milk, pasteurize it at 62° C. for 30 minutes, take out 28 g after cooling, mix it with the bifidobacterium cells obtained by centrifugation, and stir evenly. The initial concentration of bifidobacteria in the skimmed milk suspension is about 1-2×10 10 cfu / ml.

[0043] (4) Cool 30 g of the skimmed milk bacteria suspension to 4° C., add 200 μl of chymosin and let stand at 4° C. After a while add 200 μL of 10% (W / V) CaCl 2 .

[0...

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Abstract

The invention discloses a method for preparing micro-encapsulation bifidobacteria, which adopts skimmed milk as a wall material source for a phase separation coagulation method, and forms a microcapsule through the interaction between chymosin and lactoprotein. The technical proposal of the method comprises that: the fat-milk concentration is between 20 and 40 percent, the chymosin acting time is between 30 and 120 minutes, the vegetable oil mixing ratio is 10:1-2:1, and the stirring rotation speed during emulsification is between 100 and 1,000 revolutions per minute. The micro-encapsulation bifidobacteria prepared by the method have the advantages of high embedding rate, suitable grain size, high enteric solubility, good safety and the like, and can effectively promote the renovation and the industrialization of a bifidobacteria micro-encapsulation preparation technique.

Description

technical field [0001] The invention relates to a bifidobacterium preparation, in particular to a bifidobacterium microcapsule preparation, and belongs to the technical field of probiotic product preparation. Background technique [0002] The physiological and health functions of bifidobacteria on the human body have gradually become known to people. However, the good probiotic function of bifidobacteria must require a large number of surviving bacteria to reach the intestinal tract through the gastric environment and be able to colonize the intestinal mucosa. In fermented dairy products, oxygen damage, gastric acid and bile salts in the gastrointestinal tract will greatly reduce the number of viable bifidobacteria, so it is of great significance to study how to effectively protect bifidobacteria. [0003] Microencapsulation is a newly emerging technology in recent years, which refers to embedding solid, liquid and gas substances into tiny dense capsules by special means. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04B01J13/02C12R1/01
Inventor 储一衫任发政张明杨海莺赵亮刘晓飞
Owner 任发政
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