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Hypertension genetic test kit

A kit and high blood pressure technology, applied in the field of genetic detection kits for high blood pressure, can solve problems such as increased sensitivity of angiotensin, increased hypertension, increased release of aldosterone, etc.

Inactive Publication Date: 2009-12-23
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the risk genotype is carried, the number of angiotensin II-1 type receptors and the affinity for ligand binding increase, thereby enhancing the response of the arterial wall to AngII, increasing the sensitivity of the body to angiotensin, and increasing the tension of the blood vessel wall , Increased aldosterone release, easy to cause water and sodium retention, increased risk of hypertension

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Use of detection kits

[0036] Step 1: Extraction of DNA template

[0037] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0038] Step 2: Electrophoretic detection and typing

[0039] Using the electrophoresis detection kit in the detection kit, the reaction system has a total volume of 25 μl, including 1 μl of DNA template with a concentration of 12.5 ng / μl, 2.5 μl of 10X PCR reaction buffer, 0.2 μl of 25mM dNTP mixture, and 1.5 μl of 25mM MgCl2 solution , 0.125 μl of 5 units / μl Taq DNA polymerase, 0.25 μl of each primer for 20 μM electrophoresis detection primer pair, and 18.675 μl of deionized water.

[0040] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 94° C. for 12 minutes, and 30 cycles of 94° C. for 30 seconds, 60° C. for 30 seconds, 72° C. for 30 seconds, and then 72° C. for 10 minutes.

[0041] Then use electrophoresis technology to perform agarose gel electrophores...

Embodiment 2

[0049] Example 2. Application of detection kits for the detection of individual genetic susceptibility to hypertension services

[0050] Step 1: DNA Extraction

[0051] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0052] Step 2: Genotyping Assays

[0053] Use the kit provided by the present invention to perform PCR amplification and electrophoresis detection on the SNP site on the ACE gene of the subject's genomic DNA, and perform fluorescence quantitative PCR on the A1166C SNP site on the AGTR1 gene and the G894T SNP site on the NOS3 gene Detect and determine the genotypes of these 3 SNP sites.

[0054]Step 3: Analysis of individual genetic susceptibility to hypertension

[0055] Through the analysis of the SNP genotype of the tested subjects, an analysis report sheet of the individual's genetic...

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Abstract

The invention discloses a kit for testing the genetic risk of hypertension. The kit comprises specific primer pairs for testing the single nucleotide polymorphic locus genotypes of an angiotensin converting enzyme gene (ACE), an angiotensin II receptor type1 gene (AGTR1) and an endothelial type nitric oxide synthase gene (NOS3) as well as specific fluorescent probe pairs, fluorescent quantitative PCR regular components, PCR reaction components and the like. The kit estimates the individual genetic risk of hypertension by synchronously testing the polymorphic locus genotypes of the genes closely related to the genetic risk of hypertension.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the genetic susceptibility of individual hypertension. The genotypes of single nucleotide polymorphisms in tensin II type 1 receptor gene (AGTR1) and endothelial nitric oxide synthase gene (NOS3) were used to evaluate individual genetic susceptibility to hypertension. Background technique [0002] Essential hypertension (essential hypertension, EH) is a disease with elevated blood pressure as the main clinical manifestation, usually referred to as hypertension. Hypertension is an important cause and risk factor of various cardiovascular and cerebrovascular diseases. It affects the structure and function of important organs such as the heart, brain, and kidneys, and eventually leads to the failure of these organs. It is still the main cause of death from cardiovascular diseases one. [0003] The prevale...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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