Culture method of phobotaxis insect resisting rice
A cultivation method and rice technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as lack of resistance, food safety and ecological safety disputes, and achieve the effect of easy acceptance and promotion
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Embodiment 1
[0018] Example 1: Cultivation of evasive insect-resistant rice IRRice-1 from mutagenized plants
[0019] The two-line hybrid rice CMS line Guangzhan 63, which has the largest application area in China, was subjected to 300Gy cobalt-60 ray mutagenesis. More than 30,000 second-generation mutants were constructed in 2004 as materials, soaked to accelerate germination, and sowed in seedling fields. Before transplanting, sample the young leaves of 5-7 leaf age, measure the content of two kinds of pigments, chlorophyll a and chlorophyll b, calculate the total chlorophyll content and the ratio of chlorophyll a / chlorophyll b, and select the total chlorophyll content below 12 parts of rice materials with 3.5 mg / g and the ratio of chlorophyll a / chlorophyll b greater than 3.3 were transplanted into the field as a single plant; under the condition of no spraying, observe the longitudinal rolling of the leaves at the tillering stage, jointing stage, and heading stage The harm situation of ...
Embodiment 2
[0020] Example 2: Cultivation of evasive insect-resistant rice IRRice-2 from germplasm resources in my country
[0021] Using 2147 rice germplasm resources collected from Yunnan, Hainan, Sichuan, Hunan, Hubei, Jiangxi and Zhejiang provinces and cities in China as materials, the seeds were soaked to accelerate germination and sowed in seedling fields. Before transplanting, 5-7 leaf-age young Leaves, measure the content of chlorophyll a and chlorophyll b in the leaves, calculate the total chlorophyll content and the ratio of chlorophyll a / chlorophyll b, and select the ones with the total chlorophyll content lower than 3.5 mg / g and the ratio of chlorophyll a / chlorophyll b greater than 3.3 13 parts of rice materials were transplanted into the field according to individual plants; under the condition of no spraying, the damage of rice leaf rollers on the leaves was observed at the tillering stage, jointing stage, and heading stage. 9 parts of rice materials, observe the harm situat...
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