Culture method of phobotaxis insect resisting rice

A cultivation method and rice technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as lack of resistance, food safety and ecological safety disputes, and achieve the effect of easy acceptance and promotion

Inactive Publication Date: 2010-01-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Bt rice can effectively control lepidopteran pests like commercialized Bt cotton, but because it is a traditional staple food, potential food safety and ecological security issues have attracted much attention and controversy
On the other hand, Bt rice has a limitation that it is only resistant to Lepidoptera pests and lacks resistance to rice planthoppers, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Cultivation of evasive insect-resistant rice IRRice-1 from mutagenized plants

[0019] The two-line hybrid rice CMS line Guangzhan 63, which has the largest application area in China, was subjected to 300Gy cobalt-60 ray mutagenesis. More than 30,000 second-generation mutants were constructed in 2004 as materials, soaked to accelerate germination, and sowed in seedling fields. Before transplanting, sample the young leaves of 5-7 leaf age, measure the content of two kinds of pigments, chlorophyll a and chlorophyll b, calculate the total chlorophyll content and the ratio of chlorophyll a / chlorophyll b, and select the total chlorophyll content below 12 parts of rice materials with 3.5 mg / g and the ratio of chlorophyll a / chlorophyll b greater than 3.3 were transplanted into the field as a single plant; under the condition of no spraying, observe the longitudinal rolling of the leaves at the tillering stage, jointing stage, and heading stage The harm situation of ...

Embodiment 2

[0020] Example 2: Cultivation of evasive insect-resistant rice IRRice-2 from germplasm resources in my country

[0021] Using 2147 rice germplasm resources collected from Yunnan, Hainan, Sichuan, Hunan, Hubei, Jiangxi and Zhejiang provinces and cities in China as materials, the seeds were soaked to accelerate germination and sowed in seedling fields. Before transplanting, 5-7 leaf-age young Leaves, measure the content of chlorophyll a and chlorophyll b in the leaves, calculate the total chlorophyll content and the ratio of chlorophyll a / chlorophyll b, and select the ones with the total chlorophyll content lower than 3.5 mg / g and the ratio of chlorophyll a / chlorophyll b greater than 3.3 13 parts of rice materials were transplanted into the field according to individual plants; under the condition of no spraying, the damage of rice leaf rollers on the leaves was observed at the tillering stage, jointing stage, and heading stage. 9 parts of rice materials, observe the harm situat...

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PUM

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Abstract

The invention relates to a culture method for phobotaxis insect resisting rice, which comprises the following steps: collecting rice materials, soaking seeds and sprouting, seedling the seeds to a rice seed-bed, and measuring the contents of chlorophyll a and chlorophyll b in leaves at 5 to 7 leaf age before transplanting; selecting and remaining the rice materials the total contents of the chlorophylls of which are lower than 3.5 mg/g and the ratio of the chlorophyll a and the chlorophyll b is greater than 3.3; transplanting single plants to the field without spraying medicine, respectively observing rice leaf roller at the stooling stage, the jointing stage and the heading stage, observing striped rice borer and yellow rice borer at the heading stage, and observing the damage of rice planthopper at flowering period and the mature period; selecting and remaining the rice material insusceptible to the damage of insect pests; evaluating and verifying the total content and the ratio of the chlorophyll a and the chlorophyll b, and the stability for resistance of the rice leaf roller, the striped rice borer, the yellow rice borer and the rice planthopper; and propagating the stable materials to obtain a novel rice material with phobotaxis insect resistance.

Description

technical field [0001] The invention relates to a method for cultivating evasive insect-resistant rice. Background technique [0002] The occurrence of insect pests affects the high and stable yield and quality optimization of rice, and restricts the potential of increasing rice production. In recent years, stem borers and rice planthoppers have exploded across the country, becoming major disasters that seriously threaten the safety of rice production in my country. In order to control the harm of rice pests, due to the lack of new insect-resistant rice varieties in production, we can only rely on chemical control of pesticides, which results in increasingly serious environmental pollution and other problems. [0003] New insect-resistant rice varieties are highly valued at home and abroad because of their environmental friendliness. With the advent and development of transgenic technology, insect-resistant transgenic rice has come out one after another. Bt rice has been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 吴殿星舒小丽刘贵付叶恭银夏英武
Owner ZHEJIANG UNIV
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