Preparation method of estrogen specific nanometer silica gel surface molecule imprinting material
A nano-silica gel and molecular imprinting technology, which is applied in the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., can solve the problem of the decrease of the specific adsorption capacity of the target molecule, the change of the shape and the movement of the recognition site, and the influence of the adsorption amount of MIPs, etc. problems, to achieve the effect of simple and easy-to-obtain experimental equipment, easy to repeat, save time and reagents
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Embodiment 1
[0028] At room temperature, 2ml of mass concentration of 25% ammonia and 2ml of double distilled water were dissolved in 50ml of absolute ethanol, and 2.5ml of tetraethyl orthosilicate (TEOS) was added dropwise to the above mixture at a rate of 0.025ml. min -1 , 200r·min -1 The reaction was stirred for 24h. The obtained silica sol nanospheres were washed with absolute ethanol until neutral for later use.
[0029] Disperse 100mg of silica sol nanospheres with a particle size of 150nm in 50ml of toluene, add 2ml of γ-methacryloxypropyltrimethoxysilane, and react under nitrogen protection at 120°C for 16 hours. Toluene was washed 4 times by ultrasonic centrifugation at 16500r / min to obtain modified silica sol nanospheres;
[0030] Functional monomer methacrylic acid, template molecule estradiol, crosslinking agent ethylene glycol dimethacrylate and initiator azobisisobutyronitrile are dissolved in 50 milliliters of porogen toluene to make the above functional monomer The mola...
Embodiment 2
[0033] At room temperature, 2ml of mass concentration of 25% ammonia and 2ml of double-distilled water were dissolved in 50ml of absolute ethanol, and 2.5ml of tetraethyl orthosilicate (TEOS) was added dropwise to the above mixture at a rate of 0.025ml. min -1 , 200r·min -1 The reaction was stirred for 24h. The obtained silica sol nanospheres were washed with absolute ethanol until neutral for later use.
[0034] Disperse 50mg of silica sol nanospheres with a particle size of 150nm in 50ml of toluene, add 1ml of γ-methacryloxypropyl trimethoxysilane, and react under nitrogen protection at 120°C for 12 hours. Acetonitrile was ultrasonically washed three times at 16500r / min to obtain modified silica sol nanospheres;
[0035] Dissolve functional monomer methyl methacrylate, template molecule estradiol, crosslinking agent ethylene glycol dimethacrylate and initiator azobisisobutyronitrile in 50 milliliters of porogen acetone to make the above functional The molar concentration...
Embodiment 3
[0038] At room temperature, 2ml of mass concentration of 25% ammonia and 2ml of double-distilled water were dissolved in 50ml of absolute ethanol, and 2.5ml of tetraethyl orthosilicate (TEOS) was added dropwise to the above mixture at a rate of 0.025ml. min -1 , 200r·min -1 The reaction was stirred for 24h. Gained silica sol nanospheres were washed with absolute ethanol until neutral for later use.
[0039] Disperse 150mg of silica sol nanospheres with a particle size of 150nm in 50ml of toluene, add 2ml of γ-methacryloxypropyl trimethoxysilane, nitrogen protection, 120°C high temperature reaction for 12 hours, the reaction product is water, acetone Ultrasonic centrifugation and washing at 16500r / min respectively for 5 times to obtain modified silica sol nanospheres;
[0040] Functional monomer acrylamide, template molecule estradiol, crosslinking agent ethylene glycol dimethacrylate and initiator azobisisobutyronitrile are dissolved in 50 milliliters of porogen acetonitril...
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