Method for heightening salt tolerance and drought tolerance of cotton by polymeric stress-resistant gene

A drought tolerance and gene technology, applied in the field of bioengineering breeding of crops, can solve problems such as salt tolerance and drought tolerance that have not been seen

Inactive Publication Date: 2010-01-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of transgenic technology to create salt- and drought-tolerant cotton is still in its infancy at home and abroad.
So far, there have been no reports at home and abroad to obtain transgenic cotton with greatly improved salt tolerance or / and drought tolerance and practical value, and it has not been seen that betA, PIS, and Ppase related to stress resistance related to different metabolic pathways of cells are constructed into a plant to express The report of improving the salt and drought tolerance of cotton by transferring it into cotton

Method used

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  • Method for heightening salt tolerance and drought tolerance of cotton by polymeric stress-resistant gene
  • Method for heightening salt tolerance and drought tolerance of cotton by polymeric stress-resistant gene
  • Method for heightening salt tolerance and drought tolerance of cotton by polymeric stress-resistant gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Create an excellent cotton breeding material resistant to salt and alkali

[0046] 1. Construction of plant expression vectors

[0047] The target gene and the selectable marker gene of the transgenic plant are inserted into the plant transgenic expression vector through multi-step recombination using conventional molecular biology methods. For the construction process of the plant expression vector pCAMBIA1302-EPSP-betA-PIS-TsVP, see figure 1 . In this example, the target gene is the choline dehydrogenase gene betA from Escherichia coli, the pyrophosphatase gene Ppase (TsVP) on the tonoplast membrane from Salmus japonica, and the key enzyme of the phosphoinositide signaling pathway from corn Phosphatidylinositol synthase PIS gene. The selectable marker gene is the herbicide resistance gene EPSP (EPSPS: Enolpyruvylshikimate-3-phosphate synthase)

[0048] The specific recombination process is as follows: the plant expression vector pCAMBIA1302-EPSP-betA is...

Embodiment 2

[0063] Example 2: Creating excellent cotton breeding materials with significantly improved drought tolerance

[0064] 1. Construction of plant expression vectors

[0065] The construction of the plant expression vector is the same as that of the plant expression vector in Example 1.

[0066] 2. Acquisition of cotton transgenic plants

[0067] The acquisition of cotton transgenic plants is the same as that of the cotton transgenic plants in Example 1.

[0068] 3. Drought tolerance detection of transgenic plants

[0069] The transgenic plants are self-crossed or sister-bred to set fruit after flowering. The seeds are sown in the field or in the greenhouse. When the plants grow to the 4-6 leaf stage, the leaves are taken to extract DNA, and PCR technology is used to detect whether there is an exogenous gene. Transgenic positive plants were taken for stress resistance testing.

[0070] Transgenic cotton delinted by sulfuric acid and Lumianyan 19 (control) seeds were planted i...

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Abstract

The invention discloses a method for heightening the salt tolerance and the drought tolerance of cotton by polymeric stress-resistant gene, comprising the following steps of: recombining phosphatidyl inositol synthetase gene PIS from a signal transduction pathway of a plant, tonoplast pyrophosphatase gene Ppase isolated from an ion region, and choline dehydrogenase gene betA which is from colon bacillus and gives glycine betaine synthesis capability into a plant expression carrier; transplanting the recombinant into cotton cells to be effectively expressed; regenerating transgene plantlet; selecting transgene homozygote with the obviously heightened salt tolerance and drought tolerance from the transgene plantlet and filial generation thereof; and breeding out new cotton germplasm with good salt tolerance and salt tolerance, so as to create new material for breeding the cotton with good salt tolerance and salt tolerance for breeding the conventional variety or hybrid variety of the cotton.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, and specifically relates to a method for improving the salt and drought tolerance of cotton by transgenic polymerization of stress-resistance-related betA, PIS, and Ppase genes related to different metabolic pathways of cells. Background technique [0002] Soil drought and salinization are important environmental factors that inhibit plant growth and reduce crop yields. 50% of the world's crop acreage is threatened by drought and salinity (Bray et al., 2000). With the increase of the world's population, the shortage of water resources and the decrease of arable land, the development and utilization of arid land and saline-alkali land is becoming more and more important. About half of my country's land area is in arid and semi-arid regions. Even major agricultural areas in non-arid regions are hit by drought from time to time. Among the 1.6 billion mu of arable land in my country,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/04
Inventor 张可炜张举仁杨爱芳
Owner SHANDONG UNIV
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