Method for measuring general flavone content in mangrove bruguiera
A determination method and technology for total flavonoids are applied in the field of determination of flavonoid content, can solve the problems of affecting the accuracy of determination results, not easy to popularize, expensive equipment and the like, and achieve the effects of less steps, complete peak shape and less interference
Inactive Publication Date: 2010-02-03
GUANGDONG OCEAN UNIVERSITY
View PDF0 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there is a problem of color stability in the photometric method, which affects the accuracy of the measurement results.
Other methods, such as high performance liquid chromatography, have the advantages of good separation effect, fast analysis speed, and high sensitivity, but the equipment is expensive, not easy to popularize, and its use is limited
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0016] Determination of total flavonoids in Lavender mangrove
[0017] (1) Wash the collected mangrove olive leaves with clear water, put them in a cool and ventilated place to dry, crush them, and use them as samples for analysis of olive leaves. Use 60% ethanol as the extractant, the ratio of solid to liquid is 1:20, extract in a water bath at 85°C for 2 hours, and repeat the extraction twice continuously, the combined extracts are separated by polyamide column chromatography, concentrated by rotary evaporation to obtain the test solution, For the determination of total flavonoid content.
[0018] (2) With rutin as the reference substance, a 10.0 mg / ml rutin stock solution was prepared with methanol, and then diluted to 1.0 mg / ml as a rutin standard working solution. Using borax buffer solution (pH=9.18) as the base solution, carry out polarographic linear scanning in the base solution of the rutin standard working solution and the test solution prepared by (1) respectively...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for measuring general flavone content in mangrove bruguiera. Rutin standard working solution is selected; borax buffer solution is used as the base solution; a polarogram is obtained by means of linear scanning; and the magnitude of peak current increases together with the increase of rutin content and takes a fine linear relation. Rutin standard working solutionswith different amounts are selected to prepare gradient series standard solutions; borax is used as the base solution; respectively measuring the peak current of the standard series solutions; a standard curve is drawn by taking the concentration of the rutin solution as a horizontal coordinate and the peak current as a vertical coordinate. A certain amount of solution which is subject to subsequent measurement and made from a mangrove bruguiera sample is selected. Under the condition of a polarogram similar to a rutin comparison product, polarographic wave of the solution to be measured is scanned, and a peak current value of the polarographic wave is measured. The concentration of the general flavone in the solution to be measured, which corresponds to the peak current value, can be found out by using the standard curve so that the general flavone content in the sample can be worked out. The method has the advantages of simple operation, stable system, wide linear scope and the like. When used for measuring the general flavone content in the mangrove bruguiera, the method can realize strong selectivity, little interference and highly accurate measurement result.
Description
technical field [0001] The invention relates to a method for determining the content of flavonoids, in particular to a method for determining the content of total flavonoids in Lavender mangrove. Background technique [0002] Flavonoids are a large class of substances that exist widely in nature and are widely distributed in the plant kingdom. Most plants can synthesize flavonoids. The structure of flavonoids is characterized by C 6 -C 3 -C 6 It is divided into different types according to the different oxidation levels of the middle pyran ring and the various substituents on the A and B rings on both sides, mainly including flavonoids, flavonols, isoflavones, and flavans. Ketones, chalcones, isoflavones, biflavones, etc. In natural plants, most flavonoids exist in the form of glycosides, and a few exist in free forms. [0003] Flavonoids have a variety of important physiological functions. Such as scavenging free radicals, anti-aging effects, antibacterial effects, i...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 范润珍
Owner GUANGDONG OCEAN UNIVERSITY