Test strip for quickly detecting antibodies in saliva for determining herpes simplex viruses

A technology for detecting herpes simplex virus and test strips, which is applied in the field of biological diagnosis, can solve the problems of unsuitable on-site detection and high cost of test strip detection, and achieve the effects of rapid response, low detection cost and high sensitivity

Inactive Publication Date: 2010-02-03
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a test strip for rapid detection of herpes simplex virus antibody saliva, the test strip can use saliva as a sample, which solves the shortcomings of the existing test strips, which are relatively expensive to detect and are not suitable for on-site detection

Method used

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  • Test strip for quickly detecting antibodies in saliva for determining herpes simplex viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of colloidal gold pads

[0022] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 0.65 mg mouse anti-human IgM antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the IgM antibody of HSV-I and HSV-II per 100 ml of colloidal gold solution, stir at room temperature for 1 hour, add 5% bovine serum albumin (BSA) by weight to block for 1 hour, centrifuge at 12,000 rpm and 4°C for 30 minutes, discard the supernatant, redissolve to 100ml with colloidal gold solution, and spread 20cm by 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0023] Coating of nitrocellulose membrane

[0024] Dissolve the human recombinant HSV-I antigen and human recombina...

Embodiment 2

[0030] Preparation of colloidal gold pads

[0031] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 1.0 mg of mouse anti-human IgM antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the IgM antibody of HSV-I and HSV-II per 100 ml of colloidal gold solution, and stir at room temperature for 1 hour, add 5% bovine serum albumin (BSA) by weight to block for 1 hour, centrifuge at 12,000 rpm and 4°C for 30 minutes, discard the supernatant, redissolve to 100ml with colloidal gold solution, and spread 40cm by 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0032] Coating of nitrocellulose membrane

[0033] Dissolve the human recombinant HSV-I antigen and human rec...

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Abstract

The invention discloses a test strip for quickly detecting antibodies in saliva for determining herpes simplex viruses, which comprises a bottom plate. A sample pad, a colloidal gold pad, a nitrocellulose membrane and a suction pad are sequentially pasted on the bottom plate; a mouse anti-human IgM antibody which is marked with colloidal gold and can be specifically combined with IgM antibodies ofHSV-I and HSV-II is attached to the colloidal gold pad; and a detection line and a quality control line are coated on the nitrocellulose membrane, wherein the detection line comprises human recombinant HSV-I and HSV-II antigens, and the quality control line can be specifically combined with the mouse anti-human IgM antibody and comprises sheep anti-mouse anti antibodies. The test strip of the invention is used for detecting the IgM antibodies of HSV-I and HSV-II in the saliva by adopting the immunochromatography technique, thereby determining whether people are infected with herpes simplex viruses or not. The test strip has the characteristics of quick reaction speed, high sensitivity, strong specificity, simple operation and lower detection cost and is suitable for self detection.

Description

technical field [0001] The invention relates to the technical field of biological diagnosis, in particular to a test strip for rapid detection of herpes simplex virus antibody saliva. Background technique [0002] Herpes simplex is caused by Herpes Simplex Virus (HSV), a DNA virus. Human herpes simplex virus is divided into two types, namely herpes simplex virus type I (HSV-I) and herpes simplex virus type II (HSV-II). Type I mainly causes infections of the skin, mucous membranes (oral mucosa) and organs (brain) other than the genitals. Type II mainly causes skin and mucous membrane infection in the genital area. These two types can be identified by fluorescent immunoassay and cell culture method. [0003] Humans are the only natural host of HSV. The virus enters the body through the respiratory tract, oral cavity, genital mucous membrane and damaged skin, and resides in the normal mucous membrane, blood, saliva and sensory ganglion cells of the human body. Most of the ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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