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Method for preparing hydrolyzed collagen protein by fresh animal skin

A technology for hydrolyzing collagen and animal skin, which is applied in the field of preparation of hydrolyzed collagen to achieve the effects of saving water resources, reducing energy consumption and production costs, and saving energy and raw materials

Active Publication Date: 2010-02-10
YANTAI LONGPU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for preparing hydrolyzed collagen from fresh animal hides, so as to overcome the aforementioned shortcomings of the existing hydrolyzed collagen preparation methods

Method used

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  • Method for preparing hydrolyzed collagen protein by fresh animal skin
  • Method for preparing hydrolyzed collagen protein by fresh animal skin
  • Method for preparing hydrolyzed collagen protein by fresh animal skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The process of preparing hydrolyzed collagen from pigskin is as follows:

[0025] (1) Preparatory work: After washing the container and pipeline, empty the container and pipeline with demineralized water.

[0026] (2) Mince fresh food-grade pigskin that has not been stored after depilation and degreasing, and requires length × width ≤ 1 × 1cm.

[0027] (3), enzymatic hydrolysis: add 1.5 tons of water and chopped pigskin with a wet weight of 110kg to the reactor, heat it to 60°C with steam under stirring, add 0.6Kg2709 protease, at this time the pH value is 8.0, heat preservation and stirring reaction 4 Hours, there is no blocky leather in the reactor at this time.

[0028] (4), removal of solid particles: the reaction solution of the (3) step reaction was left standstill for 0.7 hours, at this time the solid particles settled completely, and the solid particles and the grease layer were removed;

[0029] (5) Enzyme hydrolysis reaction-separation coupling: put the reac...

Embodiment 2

[0036] The process of preparing hydrolyzed collagen from sheepskin is as follows:

[0037] (1) Preparatory work: After washing the container and pipeline, empty the container and pipeline with demineralized water.

[0038] (2) Chopped fresh food-grade sheepskin that has been depilated and degreased without storage, requiring length × width ≤ 1 × 1cm;

[0039] (3) Enzymolysis: Add 1.5 tons of water and chopped sheepskin with a wet weight of 100kg to the reaction kettle, heat it to 40°C with steam under stirring, add 0.3Kg1398 protease, at this time the pH value is 8.2, and keep stirring for 5 hours , At this time, there is no lump leather in the reactor.

[0040] (4), removal of solid particles: the reaction solution of the (3) step reaction was left to stand for 0.5 hour, at this time the solid particles settled completely, and the solid particles and the grease layer were removed;

[0041] (5) Enzyme hydrolysis reaction-separation coupling: put the reaction solution after r...

Embodiment 3

[0049] The process of preparing hydrolyzed collagen from cowhide is as follows:

[0050] (1) Preparatory work: After washing the container and pipeline, empty the container and pipeline with demineralized water.

[0051] (2) Mince unstored fresh food-grade cowhide after depilation and degreasing, requiring length × width ≤ 1 × 1cm;

[0052] (3) Enzymolysis: add 1.5 tons of water and 120kg of chopped cowhide to the reaction kettle, heat it with steam to 65°C under stirring, add 1.2Kg2709 protease, at this time the pH value is 8.5, and keep stirring for 3 hours , At this time, there is no lump leather in the reactor.

[0053] (4), removal of solid particles: the reaction solution of the (3) step reaction was left to stand for 1 hour, at this time the solid particles settled completely, and the solid particles and the grease layer were removed;

[0054] (5) Enzyme hydrolysis reaction-separation coupling: put the reaction solution after removing solid particles and grease layer ...

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Abstract

The invention relates to a method for preparing hydrolyzed collagen protein by fresh animal skin, which mainly comprises the following steps: animal skin processing; enzymolysis; solid particulate matter elimination; enzymolysis reaction-separation and coupling; nanofiltration concentration; spray drying; and the like. The invention has high finished product yield; in addition, the average molecular weight of a product is about 2000, the product is easier to absorb by human bodies and has no bitter taste.

Description

(1) Technical field [0001] The invention relates to a method for preparing hydrolyzed collagen, which is mainly prepared from fresh animal skins. (two), background technology [0002] Hydrolyzed collagen, also known as collagen peptide, is a water-soluble small molecular protein prepared by hydrolyzing collagen. Hydrolyzed collagen has the same chemical composition as collagen. Due to the reduction of molecular weight, the solubility of hydrolyzed collagen in water is improved, so it is easier to be absorbed by the skin and hair of the human body, and its nutritional function is further exerted. At present, the main raw materials for the production of collagen are pigskin, cowhide and other animal hides, bone bonds, and fish (such as eel, dolphin, etc.) skin. [0003] Scientific research shows that hydrolyzed collagen with an average molecular weight of about 2000 (contains about 30 amino acids) and a polypeptide length of about 30-50 nanometers is most easily absorbed by t...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 张鹏贺长强
Owner YANTAI LONGPU BIOTECH
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