Method for extracting DNA from acidic mine water bed mud in coal mine

Abstract

The invention relates to a method for extracting DNA from acidic mine water bed mud in a coal mine. Buffer solution elution, supersonic oscillation and membrane filtration for thallus collection are adopted in the method, and cell lysis is performed to extract DNA. The extracted DNA can be applied to such molecular biology technologies as PCR amplification, DGGE and TRFLP and the like for analyzing microbial communities in the acidic mine water bed mud in the coal mine without purification. The method has the advantages of being simple and rapid, and being capable of truly reflecting diversityof different microbial populations in the acidic mine water bed mud in the coal mine.

Description

technical field

[0001] The invention relates to the extraction and purification of DNA, in particular to a method for extracting DNA from acid mine bottom mud in coal mines, and belongs to the technical field of molecular biology. technical background

[0002] During the mining process of coal mines, due to changes in the environment of sulfides (mainly pyrite) contained in coal-bearing strata, redox reactions occur spontaneously, which can lead to acid mine drainage. Acid mine drainage has the characteristics of low pH value and high salinity, and is highly soluble and erosive. This kind of mine drainage can carry a large amount of heavy metals and harmful chemicals into the environment. Some harmful substances in coal mine acid wastewater will also be deposited in the river sediment, and the long-term release of various pollutants in the sediment will also affect the water quality of the downstream water body. At present, many studies have been carried out on the influenc...