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Preparation method of high purity ganoderma polysaccharide

A technology of Ganoderma lucidum polysaccharide and high purity, applied in the field of preparation of high-purity Ganoderma lucidum polysaccharide, can solve the problems of inconvenient recovery, unsuitable for large-scale processing, and high cost

Active Publication Date: 2010-02-17
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The decolorization methods used for Ganoderma lucidum polysaccharides mainly include activated carbon adsorption and oxidation, but these two methods often lead to the loss of polysaccharides; most of the deproteinization methods use the Savag method, which uses a large amount of organic solvents and is more toxic. , and the recycling is inconvenient and the cost is high, so it is not suitable for large-scale treatment

Method used

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  • Preparation method of high purity ganoderma polysaccharide
  • Preparation method of high purity ganoderma polysaccharide
  • Preparation method of high purity ganoderma polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of crude polysaccharides from Ganoderma lucidum fruiting bodies by water extraction.

[0022] Slice the fruiting body of Ganoderma lucidum with a slicer and crush it into small pieces with a pulverizer. Add water according to the ratio of 1kg fruiting body: 20L water and heat to boiling. The boiling lasts for 2 hours, then filter with filter cloth (100 mesh), and repeat the above steps for the filter residue After extracting once more, it is filtered, and the filtrates extracted twice are combined, concentrated, and then spray-dried to form a powder. to obtain crude polysaccharides.

Embodiment 2

[0024] 1. Raw material preparation

[0025] 1 g of Ganoderma lucidum fruiting body crude polysaccharide in Example 1 was formulated into an aqueous solution at a mass ratio of 1.5%, and the precipitated part was removed by centrifugation.

[0026] 2. Protease treatment

[0027] Add papain into the crude polysaccharide solution in an amount of 3% of the mass of the crude polysaccharide, and react at 40° C. for half an hour.

[0028] 3. Weak basic anion resin treatment

[0029] Weak basic anion resin pretreatment: Soak with 3% hydrochloric acid for 4 hours, then rinse with distilled water to neutral, then soak with 3% NaOH solution for 4 hours, then rinse with distilled water to pH7-8;

[0030] Add the crude polysaccharide solution after the above enzymatic reaction into the pretreated weakly basic anion resin column for further decolorization and deproteinization treatment, wherein 1 liter of water extraction solution is added to 1.5 kg of resin, and the flow rate is 1ml / min;...

Embodiment 3

[0035] 1. Raw material preparation

[0036] 1 g of Ganoderma lucidum fruiting body crude polysaccharide in Example 1 was formulated into an aqueous solution according to 1% mass ratio, and the precipitated part was removed by centrifugation.

[0037] 2. Protease treatment

[0038] Add papain into the crude polysaccharide solution according to 5% (relative to the mass ratio of crude polysaccharide), and react at 39° C. for 40 minutes.

[0039] 3. Weak basic anion resin treatment

[0040] Weak basic anion resin pretreatment: Soak with 3% hydrochloric acid for 4 hours, then rinse with distilled water to neutral, then soak with 3% NaOH solution for 4 hours, then rinse with distilled water to pH7-8;

[0041] The crude polysaccharide solution after the above enzymatic reaction is added to the pretreated weakly basic anion resin column for further decolorization and deproteinization treatment, wherein 1 liter of water extraction solution is added to 2 kg of resin at a flow rate of ...

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Abstract

The invention discloses a preparation method of a high purity ganoderma polysaccharide. The method comprises the following steps: extracting raw polysaccharide through water extraction method, treating with papain, treating with anionic resin, dialyzing or ultrafiltrating to collect materials with more than 10 thousands of molecular weight and finally freeze-drying to obtain the product. The content of the ganoderma polysaccharide prepared by the method can reach above 50% and the activity of the polysaccharide is high. The method has simple and practical operation requirements and is suitableto be expanded for large-scale production.

Description

technical field [0001] The invention relates to the field of medicinal fungi, in particular to a preparation method of high-purity ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum polysaccharide is one of the main medicinal ingredients in the medicinal fungus Ganoderma lucidum, which has immune regulation, anti-tumor, anti-aging, anti-hypertensive and other activities. There are more and more polysaccharide products at the beginning, but their purity is not high due to the rough extraction method and high cost of deep processing. In addition, consumers' requirements for product purity, health functions, safety and convenience are also gradually increasing. improve. [0003] The extraction method widely used at present is the water extraction method. The crude polysaccharide product after extraction contains many impurities such as pigments, proteins, and small molecular substances, which makes the polysaccharide product darker in color and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 张劲松周帅唐庆九刘艳芳杨焱贾薇唐传红冯娜刘方罗玺
Owner SHANGHAI ACAD OF AGRI SCI
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