Preparation method of human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein and application of same on preventing and curing cow mastitis

A technology of human lysozyme and fusion protein, applied in peptide/protein components, enzyme stabilization, drug combination, etc., can solve the problems of no patent disclosure, achieve low drug resistance, high activity, and enhance antibacterial and antiviral activity Effect

Inactive Publication Date: 2010-02-17
JILIN UNIV
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Problems solved by technology

[0005] In recent years, although it has been reported to clone the human lysozyme gene for genetic engineering expression and to explore the research on the expression of human lysozyme in transgenic mice, there have also been reports on the cloning and expression of the antimicrobial peptide gene and fusion with other related genes, but no human lysozyme The fusion expression product with antibacterial peptide is used for the treatment of cow mastitis, and there is no patent disclosure related to this project

Method used

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  • Preparation method of human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein and application of same on preventing and curing cow mastitis
  • Preparation method of human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein and application of same on preventing and curing cow mastitis
  • Preparation method of human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein and application of same on preventing and curing cow mastitis

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Embodiment

[0062] Preparation of rhlyz-Catesbeianin-1 fusion protein

[0063] 1. Primer design and synthesis:

[0064] According to the human lysozyme gene sequence (NC-000012.10) and the antimicrobial peptide gene sequence published on Genebank, primers were designed: P1, P2, C1, C2, T1 and T2, and 15 flexible amino acid Linkers were designed to link the human lysozyme gene and the antimicrobial peptide Catesbeianin -1 gene concatenation. All primers were synthesized by Shanghai Bioengineering Company, and the primer sequences are as follows:

[0065] Human lysozyme upstream primer P1.5′-CG GAATTC ATGAAGGTCTTTGAAAGGTGTG-3' (introduced EcoR I restriction site)

[0066] Human lysozyme downstream primer P2: 5′-TT GCGGCCGC CACTCCACAACCTTGAACA-3′(Introduce Not I restriction site)

[0067] Catesbeianin-1 upstream primer C1: 5′-TCGGAAAAGGAGAGAAG-3′

[0068] Catesbeianin-1 downstream primer C2: 5′-GTAGGAATGTCACAGTAACG-3′

[0069] Specific upstream primer T1: 5′-GAAGGTCTTTGAAAGGTG-3′

...

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Abstract

The invention discloses a human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein, also provides a gene engineering preparation method thereof, and further provides the application of a fusion protein biological agent on preventing and curing cow mastitis. A human lysozyme and antibacterial peptide Catesbeianin-1 gene is connected with a high-efficiency eukaryotic expression vector in afusion protein form through a gene recombination mode to further enhance the antimicrobial and antiviral activity of the fusion protein so as to obtain an antimicrobial and antiviral recombined protein with higher activity, and the fusion protein is used to prevent and cure the cow mastitis so as to develop a novel medicine which has no chemical medicine residue and antibiotic residue and low medicine resistance and can be used for preventing and curing the cow mastitis,.

Description

technical field [0001] The invention discloses a human lysozyme-antibacterial peptide Catesbeianin-1 fusion protein, and also provides its genetic engineering preparation method. The invention further provides the application of the fusion protein biological preparation in the prevention and treatment of cow mastitis, which belongs to veterinary biology pharmaceutical field. Background technique [0002] Cow mastitis is one of the most common diseases of dairy cows, the most difficult to prevent and cost, and has caused serious harm to the production of dairy cows. Seriously affect the quality of milk, indirectly affect human health, and bring huge economic losses to the dairy industry. [0003] At present, the commonly used drugs for the treatment of cow mastitis are antibiotics, such as ciprofloxacin, penicillin, etc., but due to the serious drug resistance caused by the long-term use of antibiotics, the proportion of coagulase-negative staphylococcus has increased in rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N15/81A61K38/47A61P15/14
Inventor 雷连成欧阳萍韩文瑜杜崇涛江丽娜吕爽
Owner JILIN UNIV
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