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Method for preparing agarose

A technology of agarose and sugars, applied in the field of polysaccharides, can solve the problems of agarose production research reports, no Neochlorisoleoabundans, industrialized culture of microalgae to produce agarose, etc., to achieve large-scale promotion, high production efficiency, and short production cycle Effect

Inactive Publication Date: 2010-02-17
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the production of agarose by industrialized cultivation of microalgae, and there is no related research report on the production of agarose by Neochloris oleoabundans

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The agarose of the present embodiment, its preparation method comprises the steps:

[0031] (1) Microalgae cultivation

[0032] In 1L of sterilized inorganic salt medium, inoculate Neochloris oleoabundans to make the number of cells reach 1×10 6 , into a 5 vol% CO 2 The air, ventilation volume 1vvm, light stirring culture at 28 ℃, light intensity 300μmol / m 2 ;Cultivation adopts the mode of purebred culture, cultivates for 60 hours, and the content of microalgae cells in the culture medium increases to OD 600 5; add 100ml of 50g / 100ml sterilized lactose solution to the culture solution, so that the sugar concentration in the culture solution reaches about 5g / l, and continue to cultivate. When the total culture time reaches 120 hours, stop the culture;

[0033] (2) Extraction of agarose

[0034] Heat the culture solution obtained from the above microalgae culture to 85°C, centrifuge (3000rpm, 15min) and collect the supernatant;

[0035] Concentrate the above supernat...

Embodiment 2

[0040] The agarose of the present embodiment, its preparation method comprises the steps:

[0041] (1) Microalgae cultivation

[0042] In 10L of sterilized inorganic salt medium, inoculate Neochloris oleoabundans to make the number of cells reach 1×10 6 , fed with 10 vol% CO 2 The air, ventilation volume 1vvm, light stirring culture at 30 ℃, light intensity 200μmol / m 2 ;Cultivation adopts the mode of purebred culture, cultivates for 60 hours, and the content of microalgae cells in the culture medium increases to OD 600 5; add 100ml of 40g / 100ml sterilized glucose solution to the culture solution, so that the sugar concentration in the culture solution reaches about 4g / l, continue to cultivate, and when the total culture time reaches about 120 hours, stop the culture;

[0043] (2) Extraction of agarose

[0044] Heat the culture solution obtained from the above-mentioned microalgae culture to 90°C, centrifuge (3000rpm, 15min) and collect the supernatant;

[0045] Concentrat...

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PUM

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Abstract

The invention discloses a method for preparing agarose. The method comprises the following steps: adopting microalgae Neochlorisoleoabundans as a raw material and culturing the microalgae to secrete and generate a plurality of agarose; and collecting a secretory product to prepare the needed agarose. The agarose is produced by industrially culturing the microalgae; compared with a method for producing the agarose by large algae, such as ocean planted gracilaria, agar, and the like, the method has short production period, high production efficiency and favorable development prospect, can carryout production by fresh water inland and is beneficial for large-scale generalization.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a preparation method of agarose. Background technique [0002] Agarose is a linear polysaccharide molecule composed of D-galactose and 3,6-endether-galactose alternately linked. It is an important biochemical reagent and has been widely used in molecular biology techniques and other biochemical separation and analysis techniques. It is an indispensable material in advanced biotechnology such as biochemical separation, gene recombination, immune analysis, and disease diagnosis. Agarose can be obtained from agar (also known as agar) by separating and removing the part of sulfur agarose. At present, the global agarose production is made of macroalgae as a raw material, and then desulfurized to obtain agarose. Southern my country mainly utilizes Gracilaria, seaweed, etc. as raw materials to extract agar, and then utilizes agar to extract agarose. [0003] Agarose is widely ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/89
Inventor 李雁群克里斯托夫·Q·兰
Owner SOUTH CHINA NORMAL UNIVERSITY