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Albumin-adriamycin nano preparation, preparing method and application thereof

A nano-preparation, albumin technology, applied in the field of medicine, can solve the problems of not having, not finding, etc.

Inactive Publication Date: 2010-02-24
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of nanoparticle has good tumor targeting effect, but it does not have the property of long circulation in the body
[0004] So far, we have not found any reports on nanoparticles with dextran and chitosan as the shell and albumin and doxorubicin as the core

Method used

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  • Albumin-adriamycin nano preparation, preparing method and application thereof
  • Albumin-adriamycin nano preparation, preparing method and application thereof
  • Albumin-adriamycin nano preparation, preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1. Dissolving bovine serum albumin (BSA) and dextran (62kDa) with deionized water, its molar feed ratio is 2: 1, after the solution is mixed evenly, its pH value is adjusted to 7.1, then the solution is frozen dry. The freeze-dried solid powder was weighed, then placed in a beaker and placed in a closed container with KBr saturated solution (the relative humidity in the container was 79%), and the albumin- Dextran covalent complex. Dissolve the albumin-dextran covalent complex with deionized water, prepare a solution with an albumin concentration of 1 mg / mL, and mix it with an acetic acid solution of chitosan (molecular weight 50 kDa), so that the mass of chitosan and albumin The ratio was 1:10, and the final concentration of albumin was 0.5 mg / mL. After the pH value of the mixed solution is adjusted, the chitosan / dextran-albumin nanoparticles can be prepared by heating at 80° C. for 1 hour.

[0016] The particle size and polydispersity coefficient of the ...

Embodiment 2

[0019] Embodiment 2. will carry out the bovine serum albumin-dextran covalent complex that obtains after 24 hours Maillard reaction is dissolved with deionized water, add the chitosan (molecular weight 50kDa) acetic acid solution of different ratios, then adjust solution pH To 5.6, prepare chitosan / dextran-albumin nanoparticles by heating at 80°C for 1 hour. The results in Table 2 show that chitosan / dextran-albumin nanoparticles can be prepared within a certain range of mass ratios of chitosan and albumin.

[0020] Table 2. Chitosan / dextran-albumin nanoparticles prepared under different mass ratios of chitosan and albumin.

[0021] Mass ratio of chitosan to albumin

Embodiment 3

[0022] Embodiment 3. will carry out the bovine serum albumin-dextran covalent complex obtained after 24 hours Maillard reaction with deionized water dissolving, add chitosan (molecular weight 50~190kDa) acetic acid solution, chitosan and white The mass ratio of the protein was 1:10, then the pH of the solution was adjusted to 5.6, and the chitosan / dextran-albumin nanoparticles were prepared by heating at 80° C. for 1 hour.

[0023] Nanoparticles prepared by this method are very stable, and the particle size hardly changes after a long time in pH 7.4PBS solution, indicating that dextran exists in the outer shell of nanoparticles, which prevents chitosan from being cross-linked due to hydrogen bonds. and the aggregation that occurred. ζ-potential analysis showed that (attached figure 2), the ζ-position of the prepared nanoparticles is similar to that of chitosan alone at low pH, indicating that chitosan and dextran together constitute the shell of nanoparticles, while the core...

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Abstract

The invention belongs to the technical field of medicine, in particular to an albumin-adriamycin nano preparation with double polysaccharide casing, a preparing method and application thereof. The nano preparation is the nano particle solution taking chitosan and glucan as the casing and taking albumin and adriamycin as the core. The preparing steps are as follows: mixing and heating chitosan andalbumin-glucan covalent compound to form the nano particle solution taking albumin as the core and taking glucan and chitosan as the casing; under the acidity condition, mixing the above nano particlesolution and doxorubicin hydrochloride solution; under certain pH value, covering adriamycin in the nano particle by diffusion. The tumor targeting ability of the albumin of the invention can improvethe curative effect of adriamycin and lower the toxic and side effect of adriamycin. The chitosan on the surface of the nano particle also has the function of resisting tumor and can strengthen immunity; glucan can prevent the nano particle from quickly clearing by macrophage so as to increase the chance for the nano particle to reach the tumor.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a doxorubicin nano-preparation and its preparation method and application. Background technique [0002] Tumor is a major disease that directly threatens human health. Many effective chemotherapeutic drugs lack selectivity or targeting to cancer cells and cancer tissues, and easily damage normal tissues and cells, thus severely limiting the application of these drugs in tumor treatment. Doxorubicin is a broad-spectrum antineoplastic drug, but severe adverse reactions have been shown at effective doses [J.M.Llovet, Journal of Gastroenterology 40(2005) 225-235], and its toxic effects are mainly produced in the heart, bone marrow and intestinal tract [G. Mazue et al., International Journal of Oncology 7 (1995) 713-726]. Studies have shown that tumor tissue has micropores with a diameter of 100nm to 1000nm, while in most normal healthy tissues, the connection gap betwe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/42A61K47/36A61K9/14A61K31/704A61P35/00A61K47/64
Inventor 姚萍戚佳宁
Owner FUDAN UNIV
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