System for detecting antibiotics residue and container thereof

A detection system and antibiotic technology, which are applied in biochemical instruments, microbial determination/inspection, bioreactor/fermenter combination, etc., can solve the problems of high cost and few types of antibiotics, and achieve the effect of simple operation and high detection sensitivity

Inactive Publication Date: 2010-03-03
BEIJING ZHIYUNDA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the antibiotic rapid detection kits on the market are imported equipment and reagents, which can detect lactam and tetracycline antibiotics, but the cost is very high, and other domestic kits can only detect lactam antibiotics. There are few types of antibiotics. Therefore, it is necessary to find a fast, accurate, and low-cost detection technology that can detect commonly used antibiotics to detect whether the antibiotics in milk exceed the standard.

Method used

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  • System for detecting antibiotics residue and container thereof
  • System for detecting antibiotics residue and container thereof
  • System for detecting antibiotics residue and container thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Glucose 5g / L

[0023] Peptone 10g / L

[0024] Agar 4g / L

[0025] Pure water 1L

[0026] Bromocresol Violet 0.01g / L

[0027] Divide the above solution into containers for sterilization, and then insert the solution at a concentration of 10 5 cfu / ml Bacillus stearothermophilus, sealed with a metal film, and refrigerated at 4-8°C for later use.

Embodiment 2

[0029] Glucose 5g / L

[0030] Peptone 10g / L

[0031] Agar 4g / L

[0032] Pure water 1L

[0033] Bromocresol Violet 0.05g / L

[0034] Divide the above solution into containers for sterilization, and then insert the solution at a concentration of 10 7 cfu / ml Bacillus stearothermophilus, sealed with a metal film, and refrigerated at 4-8°C for later use.

Embodiment 3

[0036] Glucose 5g / L

[0037] Peptone 10g / L

[0038] Agar 4g / L

[0039] Pure water 1L

[0040] Bromocresol Violet 0.1g / L

[0041] Divide the above solution into containers for sterilization, and then insert the solution at a concentration of 10 7 cfu / ml Bacillus stearothermophilus, sealed with a metal film, and refrigerated at 4-8°C for later use.

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Abstract

The invention relates to a system for rapidly, sensitively and conveniently detecting antibiotics residue in food and a container thereof, belonging to the chemical testing filed of microorganism. Thedetecting system consists of detecting microorganism and a detecting indicator, which are inoculated into a detection culture medium. The invention also provides a container for filling with the detection culture medium, wherein the container consists of an opening end and a closing end, the closing end is filled with the detection culture medium and the opening end is closed by a metal membrane.The system can rapidly, accurately and cheaply detect the antibiotics residue in food.

Description

technical field [0001] The invention relates to a fast, sensitive and convenient system for detecting antibiotic residues in food and a container thereof, belonging to the field of microbial chemical detection. Background technique [0002] In 1949, the United States first discovered that antibiotics have a growth-promoting effect on livestock and poultry. Since then, many countries have widely used antibiotics in animal husbandry. my country began to use antibiotics as feed additives in the 1950s, which played a very significant role in the prevention and treatment of animal diseases. The role has achieved obvious economic benefits. However, the non-standard use of antibiotics leads to the retention and accumulation of drugs in animals, and enters the human body and ecosystem in the form of residues, which brings harm to human health and the environment. As countries around the world pay more attention to food safety, the United States, the European Union and other countrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12M1/34C12R1/07
Inventor 田卫桑华春
Owner BEIJING ZHIYUNDA TECH CO LTD
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