Novel polymeric colloidal crystal array sensor
A colloidal crystal and array technology, applied in the measurement of phase influence characteristics, etc., can solve the problems of limited resolution, unable to give quantitative results, and increase the cost of instruments.
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Embodiment 1
[0020] Weigh 1.000g of N-isopropylacrylamide, 0.2866g of acrylic acid, 0.030g of N,N'-methylenebisacrylamide, and 0.039g of sodium lauryl sulfate, and dissolve them in 100ml of distilled water. Heating to 70°C while blowing nitrogen gas, 0.06g of ammonium persulfate was added after 60 minutes to initiate polymerization. After reacting for 4 hours, poly(N-isopropylacrylamide-co-acrylic acid) microgel was obtained. Purified by dialysis in distilled water for 7-30 days. Then concentrate to 4wt%.
[0021] Take 2 g of concentrated microgel, add 0.1 g of acrylamide, 0.005 g of N, N'-methylenebisacrylamide, and 7.7 μL of 10% 2,2-diethoxyacetophenone (DEAP) in DMSO solution, fully Mix and pass nitrogen gas for 15 minutes. Stand still for 1 hour until the crystals precipitate, and irradiate with 365nm ultraviolet light for 1 hour to obtain a polymeric colloidal crystal array film of poly(N-isopropylacrylamide-co-acrylic acid) microgel.
[0022] Dissolve 0.466g of 3-aminophenylboron...
Embodiment 2
[0024] The polymeric colloidal crystal array film obtained in Example 1 was soaked in 0.050 M pH 8.5 phosphate buffer solution. The reflectance spectrum of the film was measured at different temperatures with a fiber optic spectrometer, such as figure 1 (above) shown. The polymeric colloidal crystal array film has an obvious reflection peak at 520nm, which is caused by Bragg diffraction. The specific position of the peak is mainly determined by the distance between the diffraction planes and the angle between the incident light and the diffraction plane. Since these two conditions remain unchanged, when the temperature rises from 6°C to 25°C, the position of the Bragg diffraction peak hardly changes, but the intensity increases with the change of temperature, and is consistent with the change of temperature linear relationship. At low temperature, such as 6°C, the microgel is highly swollen and contains a large amount of water, so that the difference in refractive index (die...
Embodiment 3
[0026] The polymeric colloidal crystal array membrane obtained in Example 1 was soaked in 0.050M pH7.4 phosphate buffer solution containing different concentrations of glucose at 37°C. The reflectance spectrum of the thin film is measured with a fiber optic spectrometer, such as figure 2 shown. Similarly, the position of the Bragg diffraction peak of the polymerized colloidal crystal array film did not change significantly. The binding constant of glucose to the phenylboronic acid group at pH 7.4 is small, so the Bragg diffraction intensity of the polymeric colloidal crystal array does not change much.
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