Fast sieving method for dihydropyridine blood-pressure lowering compound mixed in Chinese medicinal herb blood-pressure lowering product

Abstract

The invention relates to a fast sieving method for a dihydropyridine blood-pressure lowering compound mixed in a Chinese medicinal herb blood-pressure lowering product. The fast sieving method comprises the following steps of: (1) sampling, crushing, adding acetone-ethanol solution with volume ratio to a sample of equal to 5 : 1 and mass of 5 times to 10 times of the sample, shaking for dissolution, filtering and keeping filtering solution; (2) taking out 1ml of the filtering solution obtained from step (1), adding with 3 drops to 5 drops of potassium hydroxide-ethanol solution with mass concentration of equal to 2 percent to 3 percent, shaking evening and observing the change of color; in the case that the solution is changed into reddish-brown, which shows the dihydropyridine blood-pressure reducing compound is doped in the sample; and the dihydropyridine blood-pressure reducing compound is one or more than two of nifedipine, nitrendipine and nimodipine. The invention also disclosesa special kit for applying the method and the kit comprises the acetone-ethanol solution with volume ratio of 5 to 1 and the potassium hydroxide-ethanol solution with mass concentration of equal to 2percent to 3 percent. The sieving method is characterized by fastness, low cost, simple operation and the like.

Description

technical field [0001] The invention relates to the detection of non-biological materials by using a chemical indicator, in particular to a detection method of dihydropyridine antihypertensive compounds. Background technique [0002] Antihypertensive western medicine can be roughly divided into five categories according to their different pharmacology, diuretics, beta blockers, calcium antagonists (CCB), angiotensin converting enzyme inhibitors (ACEI) and angiotensin II receptor antagonists (ARB). Long-term overdose of antihypertensive western medicine will bring serious side effects, such as: lip numbness, headache, gastrointestinal bleeding, decreased or even loss of liver function, induction or exacerbation of diabetes. [0003] Under the lure of huge economic interests, some unscrupulous manufacturers took advantage of consumers' eagerness for treatment and the misunderstanding of the non-toxic and side effects of traditional Chinese medicine, and adulterated a large am...

AI Technical Summary

Problems solved by technology

[0007] The identification method of nifedipine and nitrendipine in the Pharmacopoeia uses acetone as the solvent, and sodium hydroxide and potassium hydroxide are easy to precipitate in the acetone solution. The experiment is carried out according to the identification method recorded in the Pharmacopoeia, and the experimental results described can not be obtained.

And the differential method of nimodipine in Pharmacopoeia selects ethanol as solvent, well-known, sodium hydroxide and potassium hydroxide are soluble in ethanol, but nifedipine, nitrendipine and nimodipine are almost insoluble in ethanol, therefore, simple Solvent replacement (ethanol instead of acetone) also failed to detect nifedipine, nitrendipine, and nimodipine

On the other hand, even if the purity of the medicine is as high as more than 90% (the purity used by the identification method recorded in the Pharmacopoeia), the color presented by the reaction in 20% sodium hydroxide-water solution is also very light, not to mention the The content of nifedipine, nitrendipine and nimodipine is low (less than 10%), and its reaction in 20% sodium hydroxide-water solution is less sensitive, and the color change is less obvious, and the extraction of Chinese herbal antihypertensive products The liquid generally has a darker color (brownish yellow), and the color change of the reaction is often covered, so it is impossible to judge the test result

Furthermore, 20% sodium hydroxide-water solution is a high-concentration strong alkaline solution, which is highly corrosive and extremely inconvenient to store and use

Therefore, the identification method recorded in the Pharmacopoeia is not suitable for detecting whether nifedipine, nitrendipine and nimodipine are adulterated in Chinese herbal antihypertensive products