Rose containing flavone, and method for production thereof

A technology of flavonoids and Chinese roses, which is applied to roses containing flavonoids and its production field, and can solve the problems of failing to accumulate flavonoids

Inactive Publication Date: 2010-03-24
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the rose that accumulates flavonoids has not been obtained through hybridization or genetic recombination.

Method used

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  • Rose containing flavone, and method for production thereof
  • Rose containing flavone, and method for production thereof
  • Rose containing flavone, and method for production thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Example 1: Simulation of the co-coloring effect of flavonoids on anthocyanins

[0108] In order to simulate the co-coloring effect of flavonoids on anthocyanins, anthocyanins were first prepared. Cyanin is extracted and purified from the petals of the Chinese rose variety "Rote Rose" (rose cv. "Rote Rose"). Delphin will be obtained from the verbena variety "Tapien Violet" (vberbena cv. "Tapien Violet" or vberbena variety Sunmaref TP-V ("Tapien Violet") ("Tapien" is a Trade Mark registered in Japan) ) in the petals of the pigment extracted by alkaline hydrolysis and purification. Malvin and Luteolin 7-O-glucoside were purchased from Funakoshi Co., Ltd., Japan.

[0109] Among the various anthocyanins thus prepared, flavonoids (Luteolin 7-O-glucoside (Luteolin 7-O-glucoside)) were prepared at pH 5.0 buffer at 0, 1, 2, 4 Equivalent molar concentration ratio was added, and the absorption spectrum was measured. As anthocyanins, Cyanin (Cyanidin 3,5-diglucoside (Cyanidin 3...

Embodiment 2

[0118]Example 2 (reference example): Introducing the F3'5'H#40 gene from pansy and the flavone synthase gene from perilla into the rose variety "Lavande"

[0119] The plasmid pYFS3 containing the flavone synthase gene from perilla described in Japanese Patent Application Laid-Open No. 2000-279182, pYFS3 was digested with XbaI, blunted at the end, and further digested with BamHI to obtain perilla flavone synthase of about 1.8 kb Gene fragment. On the other hand, pSPB906 described in WO2005 / 017147 was digested with XhoI, blunted at the end, and further digested with BamHI. Between this blunt end and the BamHI cleavage site, insert the aforementioned perilla flavone synthase gene fragment to obtain 906-pYFS3. 906-pYES3 is in the El 2 There is a perilla-derived flavone synthase gene between the 35S promoter and the D8 terminator (both described in WO2005 / 017147).

[0120] The BamHI and SalI sites of pSPB176 described in the report of Ueyama et al. (Ueyama et al. Plant Science, 1...

Embodiment 3

[0129] Example 3: Introducing the F3'5'H#40 gene from Pansy and the flavone synthase gene from Butterfly into the rose variety "Lavande"

[0130] The pSPB426 was obtained by inserting the Butterfly-derived flavone synthase gene into the EcoRI and XhoI sites of plasmid pBluescript IISK(-) described in the report by Akashi et al. (Plant Cell Physiol 40, 1182-1186, 1999). After digesting it with KpnI, the end was smoothed, and further digested with BamHI to obtain a flavone synthase gene fragment of about 1.7 kb from Papilionaceae. On the other hand, pSPB906 described in WO2005 / 017147 was digested with XhoI, blunted at the end, and further digested with BamHI. Between the blunt end and the BamHI cutting site, the aforementioned flavone synthase gene fragment of Butterfly grass was inserted to obtain 906-426.

[0131] At the BamHI and SalI sites of pSPB176 described in the report of Ueyama et al. (Ueyama et al. Plant Science, 163, 253-263, 2002), the partial digestion with BamHI ...

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Abstract

The invention provides a rose characterized by comprising a flavone added by a genetic modification method. The flavone is typically produced by expression of a transferred flavone synthase gene. Theflavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically, the invention may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae.

Description

technical field [0001] The present invention relates to a rose artificially containing flavone. The present invention relates to a method for changing the flower color of rose by using the co-pigmentation effect produced by gene manipulation containing flavonoids, especially a method for changing the flower color to blue. Background technique [0002] Flowers are the reproductive organs of plants and are necessary for the production of seeds for the next generation. To form a seed, pollen must attach to the pistil for fertilization. Usually pollen transport is performed by bees, insects such as butterflies, hummingbirds, and occasionally bats. The role of the petals is to lure these pollinators, and the plant manages to change color, shape, and pattern as a result. [0003] When appreciating flowers, flower color is the most important trait of flowers, so flowers of various colors have been bred through hybridization from ancient times to the present. However, it is rare...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A01H5/00
CPCC12N15/825C12N9/0077C12N9/0071
Inventor 田中良和胜元幸久福井祐子水谷正子中村典子户上纯一
Owner SUNTORY HLDG LTD
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