Method for inducing cleavage gynogenesis of fish fry of cynoglossus semilaevis

Abstract

The invention discloses a method for inducing cleavage gynogenesis of fish fry of cynoglossus semilaevis, which comprises the steps of cynoglossus semilaevis sperm freezing storage, ultraviolet radiation of sperms, and induction and identification of the cleavage gynogenesis of the fish fry. The cynoglossus semilaevis sperm freezing storage method is established, and a method for inhibiting the first cleavage to induce the multiplication of embryo chromosome of the gynogenesis of the cynoglossus semilaevis through hydrostatic pressure is adopted so as to screen effective dilution and freezing method for the cynoglossus semilaevis sperm freezing storage and screen the hydrostatic pressure shock starting time, hydrostatic pressure and processing time suitable for the cleavage gynogenesis of cynoglossus semilaevis; and an identification method for the gynogenesis of the fish fry of the cynoglossus semilaevis is established. In the method, the fish fry of the cynoglossus semilaevis through cleavage gynogenesis is obtained for the first time, and the inductivity of the gynogenesis of the fish fry reaches 0.25 percent; and the method has the characteristics of advancement, high efficiency, reliability and reliability, can be used for solving the problems that male cynoglossus semilaevis grows slowly, the ratio of female cynoglossus semilaevis is low and the culture cost is high, and has significant application value and wide promotion and application prospect in the aspects of breeding of cynoglossus semilaevis fry, sex control, culture of all female fry, pure line breeding and the like.

Description

Technical field: [0001] The technology belongs to the field of fish biotechnology, and is a method for inhibiting the first cleavage of gynogenetic eggs of half-smooth tongue sole and obtaining diploid fry of cleavage gynogenetic eggs. Background technique: [0002] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my country, mostly in the Yellow Sea and Bohai Sea. Semi-smooth tongue sole is one of the main species of seawater fish farming at present. Because of its delicious taste, tender meat and rich nutrition, it is very popular among consumers. Its market value is extremely high, and its breeding prospect is very broad. [0003] In view of the breeding potential and potential economic and social benefits of tongue sole, in recent years, Chinese scientific and technological workers have carried out a lot of research ...

AI Technical Summary

Problems solved by technology

Therefore, the research on fish cleavage gynogenesis has received great attention internationally. Due to the difficulty in inducing cleavage gynogenesis and the low survival rate of diploids, although the research has been carried out for nearly 20 years, the relevant fish egg The research on split gynogenesis is only done on red sea bream (Kato et al., 2002 Aquaculture in Japan), flounder (Yamamoto in Japan, 1999 Aquaculture) and perch (Francescon et al. in Italy, 2004 Aquaculture). It has been reported that these types of fish are XY sex-determined fish

However, there have been no reports on the cleavage gynogenesis of the ZW sex-determining type Semi-smooth tongue sole

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