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Plant drought resistance and salt tolerance associated protein EeABF6, coding gene and application thereof

A related protein and salt-tolerant technology, applied in the field of genetic engineering, can solve the problems of inability to achieve plant stress resistance and small improvement

Inactive Publication Date: 2013-03-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tolerance of plants to adversity stresses such as drought and high salinity is a quantitative trait controlled by multiple genes, and its physiological and biochemical processes are regulated by gene interaction and co-regulation. Although the introduction of a single functional gene can improve the stress resistance of plants to a certain extent, However, the improvement range is not large, and the purpose of effectively enhancing plant stress resistance is often not achieved (Liu et al., 1998; Liu Qiang et al., 2000)

Method used

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  • Plant drought resistance and salt tolerance associated protein EeABF6, coding gene and application thereof
  • Plant drought resistance and salt tolerance associated protein EeABF6, coding gene and application thereof
  • Plant drought resistance and salt tolerance associated protein EeABF6, coding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0087] Example 1: cDNA cloning of EeABF6 gene related to drought resistance and salt tolerance of E. elongatum.

[0088] The 45-day-old E. elongatum seedlings were subjected to drought treatment for 5 hours, and Trizol was used to extract the total RNA of E. elongatum. 5'RACE kit (5'RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3'RACE kit (3'RACE System for Rapid Amplification of cDNAEnds Kit) (GIBCOBRL, CAT .NO.18373-019) to obtain the EeABF6 gene.

[0089] Total RNA was extracted from Echinopsis elongatum seedlings with Trizol, and cDNA was obtained by reverse transcription with superscript II (invitrogen) reverse transcriptase. Primers P1 and P2 were designed according to the sequence of the coding region of EeABF6 gene. Using the cDNA obtained by reverse transcription as a template, PCR amplification was performed with primers P1 and P2. The sequences of primers P1 and P2 are as follows:

[0090] P1: 5'-GCGGGATGGACTTTCCGG-3',

...

Embodiment 2

[0094] Embodiment 2: Cultivate drought-resistant, salt-tolerant transgenic plants with EeABF6 gene

[0095] 1. Construction of recombinant expression vector

[0096] 1) Construction of 35S-EeABF6 recombinant expression vector

[0097] Use the cDNA obtained by reverse transcription of the total RNA of Echinopsis elongatum as a template, and perform PCR amplification with specific primers containing SmaI and XbaI adapter sequences; then SmaI and XbaI double-enzyme digest the PCR product, recover it, and forward Insert the vector pBI121 between the SmaI and XbaI restriction sites behind the CaMV 35S promoter to obtain the recombinant vector 35S-EeABF6.

[0098] The primer sequences are as follows:

[0099] EeABF6[SmaI]5'-GCGCCCGGGGCGGGATGGACTTTCCGG-3'

[0100] EeABF6[XbaI]5'-TGCTCTAGACCAAGGGCCCGTCAGCGT-3'

[0101] 2) Construction of 29A-EeABF6 recombinant expression vector

[0102] Use the cDNA obtained by reverse transcription of the total RNA of Echinopsis elongatum as a t...

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Abstract

The invention relates to the field of genetic engineering, more specifically to a plant drought resistance and salt tolerance associated protein EeABF6, a coding gene and application thereof. The protein has an amino acid sequence shown in SEQ ID NO.1 and a gene sequence shown in SEQ ID NO.2. The drought resistance and salt tolerance associated protein and the coding gene thereof have important theoretical and practical significance for improving / enhancing the stress resistance of tobacco, increasing yield, accelerating the process of stress-resistant molecular breeding and effectively saving water resources.

Description

technical field [0001] The present invention relates to the field of genetic engineering, in particular, the present invention relates to a plant drought resistance and salt tolerance related protein EeABF6 and its coding gene and application. Background technique [0002] Abiotic stresses such as drought and salinity are the main limiting factors for plant growth and development. According to statistics, the world's arid and semi-arid regions account for 33% of the earth's land area, and saline-alkali land accounts for 7.6% of the earth's land area. In my country, the area of ​​arable land in arid areas is about 570 million mu, and the saline-alkali land is about 554 million mu. Every year, due to adversity such as drought and saline-alkali, the grain output of wheat is reduced by 70-80 billion kilograms. Wheat is one of the important food crops in our country and occupies a very important position in the national economy. However, adversity stresses such as drought and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N15/82
Inventor 高世庆赵昌平唐益苗杨颖王永波
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES