Application of loop proline-3-hydroxy-2-aminobutyric acid in preventing and removing marine fouling organisms
A technology for biological control and marine fouling, applied in the field of marine antifouling coatings, to achieve the effects of high safety, inhibition of marine biological adhesion, and good social benefits
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Embodiment 1
[0027] Experimental group I: The ring (proline-threonine) was dissolved in methanol to prepare a solution with a concentration of 28.26 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the ring (proline-threonine) coated on the bottom of the petri dish is 1 μg / cm 2 . Add 10ml of sea water.
[0028] Experimental group II: The ring (proline-threonine) was dissolved in methanol to prepare a solution with a concentration of 183.69 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the ring (proline-threonine) coated on the bottom of the petri dish is 6.5μg / cm 2 . Add 10ml of sea water.
[0029] Control group: Add 1ml of methanol to distribute the solution evenly on the bottom of the petri dish, and then ad...
Embodiment 2
[0036] Experimental group: the ring (proline-threonine) was dissolved in methanol to prepare a solution with a concentration of 282.6 μg / ml. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the ring (proline-threonine) coated on the bottom of the petri dish is 10μg / cm 2 . Add 10ml of sea water.
[0037] Control group: Add 1ml of methanol to distribute the solution evenly on the bottom of the petri dish, and then add 10ml of seawater after the solvent has evaporated completely.
[0038] Blank group: add 10ml of sea water.
[0039] Determination of the number of larvae: Take the water body containing the larvae of Pinctada martensii from the nursery pond and concentrate it with a sieve. Take 50ml each for three times, add 1-2 drops of formalin solution to kill the larvae, and then count them under a microscope. The average value obtained is the ...
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