Method for preparing tissue engineering scaffold by pore forming of directional soluble fibres

A tissue engineering scaffold and fiber technology, applied in the field of medical materials, can solve the problems of high obturator rate, poor connection, and difficult removal of NaCl particles, etc., and achieve the effect of low obturator rate and high porosity

Inactive Publication Date: 2010-06-02
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the stent obtained by this method has a high rate of closed pores, poor connection between pores, and NaCl particles are not easy to remove.

Method used

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  • Method for preparing tissue engineering scaffold by pore forming of directional soluble fibres
  • Method for preparing tissue engineering scaffold by pore forming of directional soluble fibres
  • Method for preparing tissue engineering scaffold by pore forming of directional soluble fibres

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preparation example Construction

[0031] The preparation method of the tissue engineering scaffold provided by the invention is:

[0032] a) Making sucrose into sucrose fiber, the sucrose fiber can be spun with a rotary disk spinning device.

[0033] b) dissolving the biodegradable polymer in a solvent to prepare a polymer solution, wherein the weight ratio of the biodegradable polymer to the sucrose fiber is preferably 1:1 to 1:8, in the polymer solution, The weight-volume ratio of the biodegradable polymer to the solvent is preferably 1%-6%, and the unit of the weight-volume ratio is (g / mL)×100% known in the art.

[0034] Preferably, NaCl particles can also be added to the polymer solution, the weight volume ratio of NaCl particles to the polymer solution is preferably 1%-6%, and the particle size of the NaCl particles is preferably 100 μm-450 μm. The larger particle size of NaCl particles can create larger pores to facilitate the exchange of nutrients and metabolites by cells.

[0035] Biodegradable polym...

Embodiment 1

[0043] The polymer solution is prepared, the biodegradable polymer is selected from PLA, the solvent is selected from chloroform, and the weight of PLA accounts for 6% of the volume of chloroform.

[0044] After the sucrose is made into sucrose fibers, the sucrose fibers are spread in the moulds, and the weight ratio of PLA to sucrose fibers is 1:1. Negative pressure is applied to make the polymer solution saturate the sucrose fiber to prepare the composite solution.

[0045] The obtained composite solution was placed in a fume hood to allow the solvent to evaporate naturally, and the composite of PLA and sucrose fiber was obtained after drying.

[0046] The composite of PLA and sucrose fiber is placed in ultrapure water, and the sucrose is separated out to obtain a tissue engineering scaffold.

Embodiment 2

[0048] The polymer solution is prepared, the biodegradable polymer is selected from PLGA, the solvent is selected from acetone, and the weight of PLGA accounts for 1% of the volume of the acetone.

[0049] After the sucrose is made into sucrose fibers, the sucrose fibers are spread in the mold according to the longitudinal arrangement, and the weight ratio of PLGA to sucrose fibers is 1:8. Negative pressure is applied to make the polymer solution saturate the sucrose fiber to prepare the composite solution.

[0050] The obtained composite solution was placed in a fume hood to allow the solvent to evaporate naturally, and the composite of PLGA and sucrose fiber was obtained after drying.

[0051] The PLGA and sucrose fiber composite is placed in ultrapure water, and the sucrose is separated out to obtain a tissue engineering scaffold.

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Abstract

The invention provides a method for preparing a tissue engineering scaffold, which comprises the steps of: a) preparing sucrose into sucrose fibres; b) dissolving a biodegradable macromolecular polymer into a solvent; c) paving the sucrose fibres in a mould, and adding polymer solution prepared in the step b) into the mould; d) evaporating the solvent from complex solution prepared in the step c) to prepare a polymer and sucrose fibre complex; and e) putting the prepared polymer and sucrose fibre complex into water to separate out the sucrose. The invention also provides the tissue engineering scaffold prepared by using the preparation method. In the method for preparing the tissue engineering scaffold, the sucrose fibres are used as a pore forming agent, and the tissue engineering scaffold prepared by introducing a fibre pore forming method has a fibrous porous structure with directional arrangement and run-through interconnection, and is low in closed porosity and high in porosity.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a tissue engineering scaffold and a preparation method thereof. Background technique [0002] Tissue engineering scaffolds can better simulate the extracellular matrix, provide cells with a three-dimensional space for the exchange of nutrients and metabolites, and also act as a template, which is conducive to cell adhesion, proliferation and differentiation, guides tissue regeneration and controls tissue structure. , and is widely used in skin, blood vessel, bone, cartilage, heart and other tissue engineering research. [0003] Scaffolds used in tissue engineering research must have certain characteristics, such as excellent mechanical properties to carry mechanical loads, good biocompatibility so that cells can adhere and grow smoothly, appropriate pore size, high porosity and high mutual The connected structure enables cells to enter the scaffold smoothly and exchange nutrient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/56A61L27/58
Inventor 章培标王宗良陈学思高战团赵超王宇崔立国
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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