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Method and device for performing microalgae culture experiment by using LED dimmable light

A technology for microalgae cultivation and experimental equipment, which is applied in the field of equipment for microalgae cultivation experiments, can solve the problems of inability to adjust the optimal environment for microalgae growth, high power consumption, and low efficiency, and achieve light efficiency and stable longevity. The effect of less heat generation and high light efficiency

Inactive Publication Date: 2010-06-09
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High power consumption, high heat generation and low efficiency
The light in this incubator cannot be adjusted to the optimal environment for the growth of microalgae, therefore, it does not have the experimental function of configuring the light source for later scale production

Method used

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  • Method and device for performing microalgae culture experiment by using LED dimmable light
  • Method and device for performing microalgae culture experiment by using LED dimmable light
  • Method and device for performing microalgae culture experiment by using LED dimmable light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A device for microalgae cultivation experiments with LED dimmable light.

[0024] exist figure 1 Among them, the device is composed of a box body 1, a ventilation and light-shielding board 2, an LED control circuit 4, a fluorescent lamp control circuit 6, a temperature control circuit 12 in the box, an LED lighting assembly 3 and a fluorescent lamp lighting assembly 5. In the box body 1 , the whole box body is divided into upper and lower parts by a ventilating and light-shielding plate 2 . The ventilation and light insulation board 2 is composed of a louver metal sheet structure. The upper half of the box 1 is used for LED lighting cultivation, and the lower half of the box 1 is used for fluorescent tube control cultivation. A light-emitting component 3 consisting of 100 high-brightness LEDs with a diameter of 5mm in each of red, green, blue, yellow and white is installed on both sides of the upper half. The peak wavelengths are: red 650nm, green 520nm, blue 440nm, y...

Embodiment 2

[0027] A method for carrying out microalgae cultivation experiments with the device described in Example 1.

[0028] (1) Algae species: Chaetoceros gracilis microalgae were selected as the experimental object, and the algae species were obtained from the algae species bank of the School of Fisheries, Ocean University of China.

[0029] (2) Selection of lighting scheme: take blue LED (B) + green LED (G) spectral combination, and the control light source is fluorescent lamp. The light cycle of the two light sources is 18 hours on and 6 hours off. The light intensity is controlled at 4000Lx.

[0030] (3) Cultivation method: After seawater is filtered, it is boiled and sterilized, and after cooling, a nutrient solution is prepared, and the culture solution uses F / 2 nutrient salt formula. The salinity is 25‰, pH7.5, temperature 25.5±0.5°C, shake the flask three times a day, set three replicate samples for each experimental group, and take the average of the measurement results.

...

Embodiment 3

[0036] A method for carrying out microalgae cultivation experiments with the device described in Example 1.

[0037] The algae species were Platymonas subcordiformis microalgae, which were obtained from the Algae Species Bank of the Fisheries College of Ocean University of China. Illumination scheme selection: blue LED (B) + red LED (R) spectrum combination, and the contrast light source is fluorescent lamp. The photoperiod of the two light sources is 18 hours on and 6 hours off, and the light intensity is controlled at 4000Lx. The cultivation method and measurement method are the same as in Example 2.

[0038] Measurement results: see the attached cell density curves for blue-red (BR) LED light source and fluorescent light growth cell density curve Figure 4 . The growth rate under the BR light source was 0.418; while the growth rate under the fluorescent light source was 0.363. This shows that for the subcardioid algae under the same light intensity and light cycle, the ...

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Abstract

The invention discloses a device for performing a microalgae culture experiment by using LED dimmable light and a method for culturing microalgae by using the device. The upper half in a box body of the device is provided with a luminescence component consisting of red, green, blue, yellow and white LEDs; the lower half in the box body is provided with a fluorescent lamp luminescence component; and the upper half is isolated from the lower half by a ventilation lightproof plate. The LEDs with the five colors are connected with a single-chip control circuit respectively and the LED luminescence is controlled by adopting a PWM (pulse width modulation) technique. The manual control and the automatic control on the continuous light intensity can be selected respectively, the light spectrum and the illumination cycle can be selected, and a full-day automatic control illumination mode for simulating sun light gradual changes can be selected. Through the contrast culture with a fluorescent lamp, the optimal LED illumination scheme aiming at different microalgae can be determined. The device provides an LED illumination environment for the scale microalgae culture. The device is also suitable for scientific experiment work such as the tissue culture, the seedling culture and the like of higher plants.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation biology, and relates to a device for microalgae cultivation experiment with LED adjustable light and a method for cultivating microalgae with the device. Background technique [0002] Microalgae are an important part of primary productivity in the biosphere, and some microalgae are important bait for aquaculture or bait for bait organisms. Some microalgae are rich in nutrients and are excellent nutrients for humans. Microalgae account for nearly 50% of plant species, and have diversity, complexity, and particularity in terms of germplasm, ecological distribution, genetic information, and biochemical composition. There are no microalgae that can be directly collected in large quantities in nature, and they all need to be cultivated through engineering. At present, there are mainly two ways of large-scale artificial cultivation: open pool and closed photobioreactor. In any case, li...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/36C12N1/12F21S8/00F21V23/00H05B35/00C12R1/89F21Y113/02F21Y113/20
CPCC12M23/34C12M21/02C12M31/10
Inventor 苗洪利王晶周晓光刘逢学李跃鹏梅浩
Owner OCEAN UNIV OF CHINA
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