Method for preparing anti-cancer total saponin extracts of oplopanax elatus nakai leaves
A technology of A. chinensis leaves and total saponins, which is applied to the preparation of high-purity A. chinensis leaf total saponins extracts and the application fields of anti-cancer drug preparation.
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Embodiment 1
[0031] Embodiment 1: the preparation method of the total saponins extract of ginseng leaves
[0032] 1. Preparation of total saponins from ginseng leaves by solvent extraction
[0033] Take 1 kg of ginseng leaf powder, extract twice with 60% ethanol under reflux, each time for 2 hours, combine the extracts, recover the ethanol solution, concentrate to a small volume (about 1500 ml), extract three times with an equal amount of water-saturated n-butanol, and combine The extract was concentrated under reduced pressure with n-butanol to obtain an extract, which was dried under reduced pressure to obtain 103 g of total saponins extract of ginseng ginseng. The total saponins were 70 g (68.0%) as measured by colorimetry, and the yield was 7.0%.
[0034] 2. Preparation of total saponins from ginseng leaves by solvent extraction
[0035] Take 1 kg of ginseng leaf powder, reflux and extract with 95% ethanol for 1 hour, combine the extracts, recover the ethanol solution, concentrate to ...
Embodiment 2
[0040] Example 2 Study on the anticancer activity of total saponins from ginseng leaves in vitro
[0041] 1. Experimental materials and reagents
[0042] Human colon cancer cell line (HCT116), human lung cancer cell line (NCI-H460), human gastric cancer cell line (MGC803), human pancreatic cancer cell line (PANC-1) and human hepatocellular carcinoma cell line (HepG2) were purchased at the Institute of Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. RPMI-1640 (product of U.S. Hyclone Company), calf serum (product of U.S. Gibco Company), tetramethyldiazolium blue (product of U.S. Biosharp Company), dimethyl sulfoxide (product of U.S. Biomol Company), 0.25% trypsin (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences)
[0043] The extract prepared by the method in Example 1 above was used in the experiment.
[0044] 2. Experimental method
[0045] The above cells were cultured in RPMI-1640 medium containing 10% calf...
Embodiment 3
[0050] Example 3 The growth inhibition experiment of total saponins of ginseng leaves on human liver cancer cells HepG2 nude mouse transplanted tumors
[0051] 1. Materials
[0052] The experimental animals are BALB / c nude mice aged 6-9 weeks, 22-30g, 50, male and female, and raised under specific pathogen-free (SPF) conditions. The total saponins of ginseng leaves were prepared according to the method in Example 1 above. Human liver cancer cells HepG-2 were purchased from the Institute of Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 5-Fluorouracil (5-FU, Hefei Huoshi Pharmaceutical Technology Co., Ltd.).
[0053] 2. Method
[0054] Preparation of total saponin solution: the total saponin extract of ginseng leaves prepared according to Example 1, accurately weigh the extract, suspend it with 0.5% CMC to prepare the required medicinal solution concentration, and shake well before administration.
[0055] Experimental method: Human l...
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