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Method for preparing anti-cancer total saponin extracts of oplopanax elatus nakai leaves

A technology of ginseng leaves and total saponins, which is applied in the preparation of high-purity ginseng leaf total saponins extract, and the application field of anticancer drug preparation

Inactive Publication Date: 2013-04-10
大连市波利盾生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant report on the preparation method of total saponins from ginseng leaves and its anticancer effect at home and abroad

Method used

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  • Method for preparing anti-cancer total saponin extracts of oplopanax elatus nakai leaves
  • Method for preparing anti-cancer total saponin extracts of oplopanax elatus nakai leaves
  • Method for preparing anti-cancer total saponin extracts of oplopanax elatus nakai leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation method of the total saponins extract of ginseng leaves

[0032] 1. Preparation of total saponins from ginseng leaves by solvent extraction

[0033] Take 1 kg of ginseng leaf powder, extract twice with 60% ethanol under reflux, each time for 2 hours, combine the extracts, recover the ethanol solution, concentrate to a small volume (about 1500 ml), extract three times with an equal amount of water-saturated n-butanol, and combine The extract was concentrated under reduced pressure with n-butanol to obtain an extract, which was dried under reduced pressure to obtain 103 g of total saponins extract of ginseng ginseng. The total saponins were 70 g (68.0%) as measured by colorimetry, and the yield was 7.0%.

[0034] 2. Preparation of total saponins from ginseng leaves by solvent extraction

[0035] Take 1 kg of ginseng leaf powder, reflux and extract with 95% ethanol for 1 hour, combine the extracts, recover the ethanol solution, concentrate to ...

Embodiment 2

[0040] Example 2 Study on the anticancer activity of total saponins from ginseng leaves in vitro

[0041] 1. Experimental materials and reagents

[0042] Human colon cancer cell line (HCT116), human lung cancer cell line (NCI-H460), human gastric cancer cell line (MGC803), human pancreatic cancer cell line (PANC-1) and human hepatocellular carcinoma cell line (HepG2) were purchased at the Institute of Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. RPMI-1640 (product of U.S. Hyclone Company), calf serum (product of U.S. Gibco Company), tetramethyldiazolium blue (product of U.S. Biosharp Company), dimethyl sulfoxide (product of U.S. Biomol Company), 0.25% trypsin (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences)

[0043] The extract prepared by the method in Example 1 above was used in the experiment.

[0044] 2. Experimental method

[0045] The above cells were cultured in RPMI-1640 medium containing 10% calf...

Embodiment 3

[0050] Example 3 The growth inhibition experiment of total saponins of ginseng leaves on human liver cancer cells HepG2 nude mouse transplanted tumors

[0051] 1. Materials

[0052] The experimental animals are BALB / c nude mice aged 6-9 weeks, 22-30g, 50, male and female, and raised under specific pathogen-free (SPF) conditions. The total saponins of ginseng leaves were prepared according to the method in Example 1 above. Human liver cancer cells HepG-2 were purchased from the Institute of Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 5-Fluorouracil (5-FU, Hefei Huoshi Pharmaceutical Technology Co., Ltd.).

[0053] 2. Method

[0054] Preparation of total saponin solution: the total saponin extract of ginseng leaves prepared according to Example 1, accurately weigh the extract, suspend it with 0.5% CMC to prepare the required medicinal solution concentration, and shake well before administration.

[0055] Experimental method: Human l...

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Abstract

The invention provides a method for preparing anti-cancer total saponin extractsof oplopanax elatus nakai leaves and application thereof in anti-cancer medicaments. The method comprises the following steps: 1) crashing the oplopanax elatus nakai leaves into coarse powder; 2) extracting the powder of the oplopanax elatus nakai leaves by using water or aqueous ethanol; filtering the obtained extracting solution, combining filtrates and concentrating the filtrates to obtain a concentrated liquor; and 3) concentrating the concentrated liquor at a reduced pressure and drying the concentrate to obtain the total saponin extracts of oplopanax elatus nakai leaves. The total saponins in the total saponin extracts of the oplopanax elatus nakai leaves prepared by the method accounts for over 20 percent of the weight of the total saponin extracts of the oplopanax elatus nakai leaves and the yield is of between 1 to 30 percent. Pharmacological tests prove that the extracts have a good anti-caner effect. The total saponin extracts of the oplopanax elatus nakai leaves can be applied alone or in combination with other medicaments to serve as a raw material medicament for preparing a medicament preparation with the anti-cancer effect. The product prepared by the method is high in purity and the method is simple and practical and suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of total saponins from ginseng leaves with anticancer effect, in particular to a preparation method for preparing high-purity ginseng leaf total saponins extract and its application in the preparation of anticancer drugs. Background technique [0002] Cancer is one of the major diseases facing human beings, and has become the second major disease threatening human life. According to the World Health Organization, the number of cancer deaths in 2004 reached 7.4 million (about 13% of all deaths), and more than 70% of cancer deaths occurred in low- and middle-income countries. Cancer deaths are projected to continue to increase worldwide, with an estimated 12 million deaths from cancer in 2030. Liver cancer, together with lung cancer, gastric cancer, colon cancer and breast cancer, has become the main cancer that causes total cancer mortality every year, and about 610,000 people die from liver cancer every ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61K36/25A61P35/00A61K127/00
Inventor 窦德强刘培培康廷国
Owner 大连市波利盾生物工程有限公司
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