A method for cultivating active substances in adventitious roots of Northeast thorns by using elicitors to increase reactor

Abstract

The invention discloses a method for improving an effective substance in reactor-cultured oplopanax elatus nakai adventitious roots by using elicitors. When the oplopanax elatus nakai adventitious roots are cultured in a reactor for 30-40 d and the biomass reaches the maximum, methyl jasmonate (200 [mu]moL / L), salicylic acid (100 [mu]moL / L) and pulullan polysaccharide (200 mg / L) are added for post-treatment for 10 d, results show that the flavone content in the adventitious roots are significantly increased and is increased from 31.00 mg / g to 45.88 mg / g, wherein when the two elicitors of methyl jasmonate (200 [mu]moL / L) and pullulan polysaccharide (200 mg / L) treat the adventitious roots, the polysaccharide contents in the adventitious roots are increased substantially, the polysaccharide contents are increased by 53.33 mg / g and 97.07 mg / g respectively, and the production qualities are increased by 0.348 g / L and 0.6865 g / L respectively. The method can effectively improve the contents of flavone and polysaccharide in the adventitious roots; with combination of a tissue culture method and through reactor adventitious root culture, the oplopanax elatus nakai adventitious roots having higher contents of flavone and polysaccharide can be obtained in a short time, and a convenient and effective method is provided for resource utilization and protection of valuable and rare oplopanax elatus nakai.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a method for cultivating active substances in adventitious roots of Northeast thorns by using elicitors to improve reactors. Background technique [0002] Northeast thorn ginseng (Oplopanax elatus Nakai), also known as sea cucumber, is a perennial deciduous shrub in the Araliaceae family. It is one of the unique plant resources in Changbai Mountain in my country. Northeast ginseng has extremely high medicinal value. Its roots, rhizomes and stems are precious Chinese medicinal materials, rich in saponins, polysaccharides, flavonoids, volatile oils, anthraquinones, fatty acids and other medicinal ingredients, which can be used to treat neurasthenia and hypotension , impotence, schizophrenia, diabetes and other diseases, and has antifungal, antidepressant, antipyretic, analgesic and anti-aging effects. In recent years, it has been found that some ingredients contained in the leaves are s...

AI Technical Summary

Problems solved by technology

Due to its high medicinal value, people over-excavated it to cause a shortage of wild resources. In artificial cultivation, sexual reproduction has a low seed setting rate and a low transplant survival rate; asexual reproduction has suspended animation, and the recovery period is long. There is a shortage of material sources, and it is easily affected by climate and chemical pesticides during the cultivation process. The output cannot be kept stable, and there is a greater risk. Therefore, it is imminent to find a method that can replace the wild and artificial cultivation of Northeast thorny ginseng, and the use of plant tissue culture Propagation and large-scale production of traditional Chinese medicine resources have become an effective and feasible development path. Plant tissue culture includes cell culture, tissue and organ culture, but one of the disadvantages of medicinal plant cell culture is that the content of active ingredients is unstable, which is mainly due to Most of the active ingredients are secondary metabolites, whose synthesis is not strong at the cell stage, and root culture can solve this problem

Images