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Low temperature fermentation inoculum for preparing straw feed

A technology for fermentation inoculum and straw feed, which is applied in the field of low-temperature fermentation inoculants, can solve the problems of no straw feed fermentation inoculum, restriction on the activity of lactic acid bacteria, etc., so as to achieve the advantages of improving nutritional value and palatability, improving fermentation quality and reducing pH value. Effect

Inactive Publication Date: 2010-06-16
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the temperature in autumn and winter is relatively low, and the activity of lactic acid bacteria, the main active factor in silage, is restricted. At present, there is no straw feed fermentation agent specifically for low temperature.

Method used

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  • Low temperature fermentation inoculum for preparing straw feed
  • Low temperature fermentation inoculum for preparing straw feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, the screening of active ingredient in fermented inoculum

[0016] Composition of R-MRS medium (g / L):

[0017]

[0018]

[0019] Crush the rice stalks from the rice-producing areas in Northeast China into 1-2 cm long pieces, add 0.5 g each of the above-mentioned rice stalks, the soil from this area, and the mixture of the above-mentioned rice stalks and soil into 10 ml of R-MRS medium Medium, anaerobic culture in a 5°C refrigerator. After 10 days, 15 μl of each of the above three types of culture solutions were respectively inoculated into 300 ul of fresh R-MRS medium, transferred once every 4 days, and subcultured continuously. The pH value of the culture medium and the production of lactic acid were measured. Select a culture whose pH value drops rapidly, produces more lactic acid and is relatively stable to changes in the external environment, and screens for active ingredients.

[0020] The culture obtained above was studied by establishing a 1...

Embodiment 2

[0021] Embodiment 2, the preparation of the low-temperature fermentation bacterial agent that is used to prepare straw feed

[0022] Lactobacillus sakei JCM 1157 and Leuconostoc inhae KCTC 3774 were purchased from the Japan Microorganism Conservation Center and the Gene Bank of the Korean Institute of Biotechnology, respectively.

[0023] 1. Preparation of low-temperature fermentation bacterial agent for preparing straw feed

[0024] Lactobacillus sakei (Lactobacillus sakei) JCM 1157 and Leuconostocinhae (Leuconostocinhae) KCTC 3774 were inoculated into R-MRS medium respectively, cultured at 10°C for 24 hours, and 79ml of the above-mentioned Lactobacillus sakei (Lactobacillus sakei) JCM The fermented liquid of 1157 is mixed with the fermented liquid of 21ml above-mentioned Leuconostoc inhae (Leuconostoc inhae) KCTC 3774, and in the mixed fermented liquid, the fermentation liquid of Lactobacillus sakei (Lactobacillus sakei) JCM 1157 and Leuconostoc inhae (Leuconostoc inhae) KCT...

Embodiment 3

[0030] Embodiment 3, the preparation of the low-temperature fermentation bacterial agent that is used to prepare straw feed

[0031] Lactobacillus sakei JCM 1157 and Leuconostoc inhae KCTC 3774 were purchased from the Japan Microorganism Conservation Center and the Gene Bank of the Korean Institute of Biotechnology, respectively.

[0032] 1. Preparation of low-temperature fermentation bacterial agent for preparing straw feed

[0033]Lactobacillus sakei (Lactobacillus sakei) JCM 1157 and Leuconostocinhae (Leuconostocinhae) KCTC 3774 were inoculated into R-MRS medium respectively, cultured at 10°C for 24 hours, and 70ml of the above-mentioned Lactobacillus sakei (Lactobacillus sakei) JCM The fermented liquid of 1157 is mixed with the fermented liquid of 20ml above-mentioned Leuconostoc inhae (Leuconostoc inhae) KCTC 3774, and in the mixed fermented liquid, the fermentation liquid of Lactobacillus sakei (Lactobacillus sakei) JCM 1157 and Leuconostoc inhae (Leuconostoc inhae) KCTC...

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Abstract

The invention discloses a low temperature fermentation inoculum for preparing straw feed, which comprises the active components of Lactobacillus sakei and Leuconostoc inhae. Various lactic acid bacteria in the low temperature fermentation inoculum for preparing the straw feed of the invention have high synergetic effect so that a stable ecological system can be formed by self, can rapidly reproduce, colonize and dominate in an unpasteurized natural raw material, rapidly generate a large amount of lactic acid, reduce the pH value of a fermentation material, improve the fermentation quality of the feed, improve the nutritive value and the palatability of the feed; and the lactic acid bacteria have safety to fed animals, simple application and low cost.

Description

technical field [0001] The invention relates to a low-temperature fermentation bacterial agent for preparing straw feed. Background technique [0002] Crop straw is an important biological resource, which has the characteristics of large quantity, wide distribution and variety. According to reports, the total annual output of various crop straws in my country is more than 700 million tons, including 230 million tons of rice straw, 220 million tons of corn straw, 1 billion tons of beans and autumn miscellaneous grains, and 100 million tons of peanuts, potato vines and beet leaves. tons, accounting for about 30% of the world's total straw production. However, these straw resources have not been rationally developed and utilized for a long time. In recent years, due to the substantial increase of crop yield and the change of fuelwood structure in rural areas, more and more straws have become waste, which not only wastes resources but also pollutes the environment. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/14C12R1/01C12R1/225
Inventor 崔宗均杨洪岩王小芬
Owner CHINA AGRI UNIV
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